Human SP3 knockout HCT116 cell lysate
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SP3 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2.
View Alternative Names
Transcription factor Sp3, SPR-2, SP3
- WB
Lab
Western blot - Human SP3 knockout HCT116 cell lysate (AB257700)
Lane 1 : Wild-type HCT 116 cell lysate 20 μg
Lane 2 : SP3 knockout HCT 116 cell lysate 20 μg
False colour image of Western blot : Anti-SP3 antibody - N-terminal staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab227856 was shown to bind specifically to SP3. A band was observed at 100 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in SP3 knockout cell line ab266879 (knockout cell lysate ab257700). To generate this image, wild-type and SP3 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-SP3 antibody - N-terminal (<a href='/en-us/products/primary-antibodies/sp3-antibody-n-terminal-ab227856'>ab227856</a>) at 1/500 dilution
Lane 1:
Wild-type HCT 116 cell lysate at 20 µg
Lane 2:
SP3 knockout HCT 116 cell lysate at 20 µg
Lane 2:
Western blot - Human SP3 knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-sp3-knockout-hct116-cell-line-ab266879'>ab266879</a>)
Predicted band size: 82 kDa
Observed band size: 100 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human SP3 knockout HCT116 cell lysate (AB257700)
Homozygous : 14 bp deletion in exon2
Complementary Products
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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