Human SPAG5 (Astrin/Deepest) knockout HEK-293T cell lysate
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SPAG5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon 2 and 2 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
View Alternative Names
Astrin, Deepest, MAP 126, Mitotic spindle associated protein, Mitotic spindle coiled coil related protein, Mitotic spindle-associated protein p126, SPAG5_HUMAN, Sperm tail protein Spag 5, Sperm-associated antigen 5, hMAP 126
- Sanger seq
Unknown
Sanger Sequencing - Human SPAG5 (Astrin/Deepest) knockout HEK-293T cell lysate (AB259151)
Allele-1 : 19 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human SPAG5 (Astrin/Deepest) knockout HEK-293T cell lysate (AB259151)
Allele-3 : Insertion of the selection cassette in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human SPAG5 (Astrin/Deepest) knockout HEK-293T cell lysate (AB259151)
Allele-2 : 2 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Astrin facilitates kinetochore-microtubule attachment participating in the mitotic spindle assembly complex. This complex ensures chromosome alignment and segregation which is critical in cellular replication. Since precise regulation of this process is necessary for genetic stability Astrin's function is indispensable. Its presence influences the structural integrity of dividing cells linking it to the stability of genetic information passed to daughter cells after division.
Pathways
Astrin operates within the spindle assembly checkpoint and cell cycle pathways. The spindle assembly checkpoint pathway ensures that cells do not progress to anaphase until all chromosomes are properly attached to the spindle apparatus. In synergy with proteins like Ndc80 and KNL1 Astrin maintains attachment integrity. Its interactions with these proteins ensure accurate chromosome segregation protecting against chromosomal instability which can lead to aneuploidy.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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