Human SPCS1 knockout HEK-293T cell lysate
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SPCS1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 2 bp deletion in exon 1.
View Alternative Names
HSPC033, Microsomal signal peptidase 12 kDa subunit, OTTHUMP00000206959, SPC1, SPC12, SPCS1_HUMAN, SPase 12 kDa subunit, Signal peptidase complex subunit 1, YJR010C-A, signal peptidase 12kDa, signal peptidase complex subunit 1 homolog (S. cerevisiae)
- Sanger seq
Unknown
Sanger Sequencing - Human SPCS1 knockout HEK-293T cell lysate (AB259152)
Allele-1 : 2 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human SPCS1 knockout HEK-293T cell lysate (AB259152)
Allele-2 : 1 bp insertion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SPCS1 supports the proper processing and maturation of secretory and membrane proteins. As a constituent of the signal peptidase complex SPCS1 participates directly in the co-translational removal of signal peptides. This functionality is integral for protein targeting and trafficking ensuring that proteins reach their intended locations within the cell or are secreted outside.
Pathways
SPCS1 is involved in the protein processing pathway within the endoplasmic reticulum. This pathway plays a role in maintaining cellular protein homeostasis. The function of SPCS1 makes it relevant to the unfolded protein response pathway indirectly influencing pathways that involve stress response and protein folding. Proteins like SEC61 which form the translocon complex work closely with SPCS1 in these pathways to facilitate protein synthesis and translocation across the ER membrane.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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