Human SPCS3 knockout HeLa cell lysate
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SPCS3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 344 bp deletion in exon1.
View Alternative Names
DKFZp564J1864, FLJ22649, Microsomal signal peptidase 22/23 kDa subunit, Microsomal signal peptidase 23 kDa subunit, PRO3567, SPC22, SPC22/23, SPC3, SPase 22 kDa subunit, SPase 22/23 kDa subunit, Signal peptidase complex subunit 3, Signal peptidase complex subunit 3 homolog, Signal peptidase complex subunit 3 homolog (S. cerevisiae), UNQ1841, YLR066W
- Sanger seq
Unknown
Sanger Sequencing - Human SPCS3 knockout HeLa cell lysate (AB259153)
Homozygous : 344 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The signal peptidase complex including SPCS3 is involved in the processing and maturation of secretory and membrane proteins. SPCS3 forms part of a larger multisubunit complex that ensures proteins assume their proper functional forms. By cleaving signal peptides SPCS3 facilitates the proper localization and function of proteins within the cell. In doing so it contributes to cellular processes that depend on effective signal peptide processing such as growth and development.
Pathways
SPCS3 plays a significant role in the protein processing pathway in the endoplasmic reticulum. It interacts with other proteins involved in this pathway such as SPCS1 and SEC61 ensuring the correct translocation and maturation of newly synthesized polypeptides. The efficient functioning of SPCS3 in these pathways is important for maintaining cellular homeostasis and supporting protein synthesis and secretion.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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