Human SQLE (Squalene Epoxidase) knockout HeLa cell lysate
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SQLE KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon1 and Insertion of the selection cassette in exon1.
View Alternative Names
ERG1, SE, Squalene monooxygenase, SQLE, Squalene epoxidase
- Sanger seq
Unknown
Sanger Sequencing - Human SQLE (Squalene Epoxidase) knockout HeLa cell lysate (AB257705)
Allele-1 : 5 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human SQLE (Squalene Epoxidase) knockout HeLa cell lysate (AB257705)
Allele-2 : Insertion of the selection cassette in exon1
Complementary Products
Primary Antibodies
AB317629
Anti-Squalene Epoxidase antibody [EPR29195-89] - BSA and Azide free
Recombinant|RabMAb
![Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] - BSA and Azide free (AB317629)](https://content.abcam.com/products/images/squalene-epoxidase-antibody-epr29195-89-bsa-and-azide-free-ab317629--western-blot-img432802.jpg)
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Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Squalene epoxidase plays an important role in maintaining sterol homeostasis by regulating the conversion of squalene an important precursor in the cholesterol synthesis pathway. This enzyme operates as a part of the endoplasmic reticulum membrane complex where it anchors and acts on substrates to produce sterol intermediates for further enzymatic actions. Its function is tightly regulated by intracellular cholesterol levels to prevent overproduction and maintain cell membrane integrity.
Pathways
Squalene epoxidase is an important component of the mevalonate pathway a fundamental route for the synthesis of cholesterol and other isoprenoids. Besides cholesterol biosynthesis it connects to the synthesis of steroid hormones and bile acids. Farnesyl pyrophosphate another key intermediate in the mevalonate pathway influences the activity of squalene epoxidase. Moreover this enzyme cooperates with subsequent enzymes like lanosterol synthase to facilitate the continuation of sterol biosynthesis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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