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AB257052

Human SQSTM1 (p62) knockout HCT116 cell lysate

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SQSTM1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 49 bp deletion in exon4.

View Alternative Names

A170, DMRV, EBI 3 associated protein p60, EBI3-associated protein of 60 kDa, EBIAP, FTDALS3, MGC127197, ORCA, OSF-6, OSIL, Osi, Oxidative stress induced like, P60, PDB 3, PKC-zeta-interacting protein, Paget disease of bone 3, Phosphotyrosine independent ligand for the Lck SH2 domain p62, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Protein kinase C-zeta-interacting protein, SQSTM 1, SQSTM_HUMAN, STAP, STONE14, Sequestosome-1, Ubiquitin-binding protein p62, ZIP, ZIP 3, p62, p62B

3 Images
Western blot - Human SQSTM1 (p62) knockout HCT116 cell lysate (AB257052)
  • WB

Unknown

Western blot - Human SQSTM1 (p62) knockout HCT116 cell lysate (AB257052)

Lane 1 : Wild-type HCT116 cell lysate (20 ug)
Lane 2 : SQSTM1 knockout HCT116 cell lysate (20 ug)
Lane 3 : HepG2 cell lysate (20 ug)
Lane 4 : HeLa cell lysate (20 ug)

ab207305 was shown to specifically react with SQSTM1 / p62 in wild-type HCT116 cells. The band observed in knockout cell line ab266871 (knockout cell lysate ab257052) lane below 55 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and SQSTM1 / p62 knockout samples were subjected to SDS-PAGE. ab207305 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

false

Western blot - Human SQSTM1 (p62) knockout HCT116 cell lysate (AB257052)
  • WB

Unknown

Western blot - Human SQSTM1 (p62) knockout HCT116 cell lysate (AB257052)

Lane 1 : Wild-type HCT116 cell lysate (20 ug)
Lane 2 : SQSTM1 knockout HCT116 cell lysate (20 ug)
Lane 3 : HepG2 cell lysate (20 ug)
Lane 4 : HeLa cell lysate (20 ug)

ab109012 was shown to specifically react with SQSTM1 / p62 in wild-type HCT116 cells. The band observed in knockout cell line ab266871 (knockout cell lysate ab257052) lane below 55 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and SQSTM1 / p62 knockout samples were subjected to SDS-PAGE. ab109012 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

false

Sanger Sequencing - Human SQSTM1 (p62) knockout HCT116 cell lysate (AB257052)
  • Sanger seq

Unknown

Sanger Sequencing - Human SQSTM1 (p62) knockout HCT116 cell lysate (AB257052)

Homozygous : 49 bp deletion in exon4

Key facts

Cell type

HCT116

Species or organism

Human

Tissue

Colon

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 49 bp deletion in exon4.

Disease

Carcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.</p>" } } }
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Product details

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257052-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HCT116 cell lysate", "number":"AB257052-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human SQSTM1 knockout HCT116 cell lysate", "number":"AB257052-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Gene name
SQSTM1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Complementary Products

Primary Antibodies

AB194721

Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

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Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB194721)

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Proteins & Peptides

AB95320

Recombinant Human SQSTM1 / p62 protein

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Cell Lines & Lysates

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Assay Kits

AB289654

Human SQSTM1/p62 ELISA Kit

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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