Human SQSTM1 (p62) knockout HEK-293T cell lysate
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(0 Publication)
- WB
Lab
Western blot - Human SQSTM1 (p62) knockout HEK-293T cell lysate (AB263770)
Lane 1 : Wild-type HEK293T cell lysate (20μg)
Lane 2 : SQSTM1 knockout HEK293T cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab109012 observed at 64 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109012 Recombinant Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker was shown to specifically react with SQSTM1 in wild-type HEK293T cells in western blot. Loss of signal was observed when knockout cell line ab255343 (knockout cell lysate ab263770) was used. Wild-type and SQSTM1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109012 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (<a href='/en-us/products/primary-antibodies/sqstm1-p62-antibody-epr4844-autophagosome-marker-ab109012'>ab109012</a>) at 1/10000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
SQSTM1 knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human SQSTM1 (p62) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-sqstm1-p62-knockout-hek-293t-cell-line-ab255343'>ab255343</a>)
Predicted band size: 47 kDa
Observed band size: 64 kDa
false
- WB
Lab
Western blot - Human SQSTM1 (p62) knockout HEK-293T cell lysate (AB263770)
Lane 1 : Wild-type HEK293T cell lysate (20μg)
Lane 2 : SQSTM1 knockout HEK293T cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab56416 observed at 62 kDa. Red - loading control ab181602 observed at 37 kDa.
ab56416 Anti-SQSTM1 / p62 antibody - Autophagosome Marker was shown to specifically react with SQSTM1 in wild-type HEK293T cells in western blot. Loss of signal was observed when knockout cell line ab255343 (knockout cell lysate ab263770) was used. Wild-type and SQSTM1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab56416 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-SQSTM1 / p62 antibody [2C11] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/sqstm1-p62-antibody-2c11-bsa-and-azide-free-ab56416'>ab56416</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
SQSTM1 knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human SQSTM1 (p62) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-sqstm1-p62-knockout-hek-293t-cell-line-ab255343'>ab255343</a>)
Predicted band size: 47 kDa
Observed band size: 62 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human SQSTM1 (p62) knockout HEK-293T cell lysate (AB263770)
Homozygous : 1 bp insertion in exon 4
Complementary Products
Primary Antibodies
AB194721
Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker
RabMAb|Recombinant|KO Validated
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB194721)](https://content.abcam.com/products/images/alexa-fluor-647-sqstm1-p62-antibody-epr4844-autophagosome-marker-ab194721--immunocytochemistry-immunofluorescence-img123315.jpg)
- 0
- 0
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
D7S820, D5S818, TH01, D16S539, TPOX, CSF1PO, D13S317
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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