Human SSBP3 knockout HeLa cell lysate
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SSBP3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 101 bp deletion in exon3.
View Alternative Names
CSDP, FLJ10355, OTTHUMP00000009976, SSBP3, SSDP, SSDP1, Sequence specific single stranded DNA binding protein, Single stranded DNA binding protein 3
- Sanger seq
Unknown
Sanger Sequencing - Human SSBP3 knockout HeLa cell lysate (AB263365)
Homozygous : 101 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SSBP3 participates in DNA replication recombination and repair mechanisms. The protein acts as a part of a multi-protein complex that includes the replication protein A (RPA) which is essential for maintaining genomic stability. This association helps in the recruitment of other proteins needed for proper DNA metabolic activities. By stabilizing single-stranded DNA SSBP3 contributes to efficient DNA processing ensuring accurate cellular function and integrity.
Pathways
Single-stranded DNA-binding protein 3 plays a role in the DNA damage repair pathway and the DNA replication pathway. It associates closely with proteins such as replication protein A (RPA) and the ataxia-telangiectasia mutated (ATM) protein which are significant in responding to DNA damage and ensuring proper DNA replication. Through these interactions SSBP3 influences DNA repair mechanisms and cell cycle progression protecting cells from genotoxic stress.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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