Human SSSCA1 knockout HEK-293T cell lysate
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ZNRD2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.
View Alternative Names
1500016H19Rik, Autoantigen p27, C184L, OTTHUMP00000232769, OTTHUMP00000233069, OTTHUMP00000233071, SSA27_HUMAN, Sjoegren syndrome/scleroderma autoantigen 1, Sjogren syndrome/scleroderma autoantigen 1, Sjogren's syndrome/scleroderma autoantigen 1 homolog, centromeric autoantigen (27kD), p27
- Sanger seq
Unknown
Sanger Sequencing - Human SSSCA1 knockout HEK-293T cell lysate (AB263368)
Allele-2 : Insertion of the selection cassette in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human SSSCA1 knockout HEK-293T cell lysate (AB263368)
Allele-1 : 1 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein acts as a critical player in the regulation of mitosis. SSSCA1 forms part of a larger multiprotein complex essential for the proper attachment of chromosomes to the mitotic spindle. By ensuring that the chromosomes align correctly SSSCA1 facilitates accurate segregation during cell division. It works closely with other components of the complex to maintain genomic stability.
Pathways
The protein influences critical cell division processes. SSSCA1 integrates into the spindle assembly checkpoint pathway ensuring the proper timing of mitosis. Within this pathway it often interacts with proteins such as BubR1 and Mad2 which are responsible for halting cell cycle progression until chromosomes are correctly attached to the spindle fibers. Its role in checkpoint pathways highlights its importance in maintaining orderly mitotic progression.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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