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AB257707

Human STAG2 (SA2) knockout HeLa cell lysate

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STAG2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon11.

View Alternative Names

Cohesin subunit SA-2, DKFZp686P168, DKFZp781H1753, FLJ25871, SCC3 homolog 2, SCC3B, STAG2_HUMAN, Stromal antigen 2, bA517O1.1

3 Images
Western blot - Human STAG2 (SA2) knockout HeLa cell lysate (AB257707)
  • WB

Lab

Western blot - Human STAG2 (SA2) knockout HeLa cell lysate (AB257707)

Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : STAG2 knockout HeLa cell lysate (20 ug)
Lane 3 : HCT116 cell lysate (20 ug)

ab155081 was shown to specifically react with SA2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265461 (knockout cell lysate ab257707) was used. Wild-type and SA2 knockout samples were subjected to SDS-PAGE. ab155081 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SA2 antibody [EPR10994(B)] (<a href='/en-us/products/primary-antibodies/sa2-antibody-epr10994b-ab155081'>ab155081</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

STAG2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human STAG2 (SA2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-stag2-sa2-knockout-hela-cell-line-ab265461'>ab265461</a>)

Lane 3:

HCT116 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 141 kDa

Observed band size: 141 kDa

false

Western blot - Human STAG2 (SA2) knockout HeLa cell lysate (AB257707)
  • WB

Lab

Western blot - Human STAG2 (SA2) knockout HeLa cell lysate (AB257707)

Lane 1 : Wild-type HeLa cell lysate (20 ug)

Lane 2 : STAG2 knockout HeLa cell lysate (20 ug)

Lane 3 : HCT116 cell lysate (20 ug)

Lanes 1-3 : Merged signal (red and green). Green - ab201451 observed at 141 kDa. Red - loading control ab8245 observed at 36 kDa.

ab201451 Anti-SA2 antibody [EPR17865] - C-terminal was shown to specifically react with SA2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265461 (knockout cell lysate ab257707) was used. Wild-type and SA2 knockout samples were subjected to SDS-PAGE. ab201451 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SA2 antibody [EPR17865] - C-terminal (<a href='/en-us/products/primary-antibodies/sa2-antibody-epr17865-c-terminal-ab201451'>ab201451</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

STAG2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human STAG2 (SA2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-stag2-sa2-knockout-hela-cell-line-ab265461'>ab265461</a>)

Lane 3:

HCT116 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 141 kDa

Observed band size: 141 kDa

false

Sanger Sequencing - Human STAG2 (SA2) knockout HeLa cell lysate (AB257707)
  • Sanger seq

Unknown

Sanger Sequencing - Human STAG2 (SA2) knockout HeLa cell lysate (AB257707)

Homozygous : Insertion of the selection cassette in exon11

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon11.

Disease

Adenocarcinoma

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
STAG2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SA2 protein also known as stromal antigen 2 is a component of the cohesin complex playing a significant role in chromosomal dynamics. With an approximate molecular weight of 152 kDa SA2 exhibits high expression in the nucleus of proliferating cells particularly during interphase. This cohesin component contributes critically to maintaining sister chromatid cohesion and ensuring proper segregation during cell division serving as a functional partner in the cohesin complex with other components like SMC1 SMC3 and RAD21.
Biological function summary

SA2 plays an important role in regulating gene expression by forming loops that connect different genomic regions. SA2 as a member of the cohesin complex also participates in DNA repair by facilitating accurate recombination enhancing genome stability. This involvement is essential for maintaining chromatin structure which influences diverse cellular processes. Its expression can be an important modulator in the dynamic environment of cellular proliferation and differentiation.

Pathways

SA2 functions integrally within the cohesin-mediated sister chromatid cohesion pathway and the DNA damage response pathway. In these pathways SA2 teams with proteins such as CTCF in insulator function regulation and BRCA1 in DNA repair mechanisms. The cohesin-SA2 interaction with these proteins ensures proper chromosomal architecture and integrity which are essential for the accurate transmission of genetic material across generations of cells.

SA2 mutations or dysregulations have been linked to Cornelia de Lange Syndrome (CdLS) and certain cancers. In CdLS SA2 mutations disrupt normal cohesin function affecting developmental processes. In cancer contexts abnormal SA2 function or expression can lead to improper DNA repair contributing to genomic instability and tumorigenesis. It is often studied in concert with other cohesin proteins and regulatory elements like NIPBL which provides insights into cohesin-related pathologies and potential therapeutic targets.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

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