Human STAM (STAM1) knockout HEK-293T cell lysate
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STAM KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon7 and 20 bp deletion in exon7.
View Alternative Names
DKFZp686J2352, HSE1 homolog, OTTHUMP00000019237, STAM, STAM1_HUMAN, Signal transducing adapter molecule 1, signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
- Sanger seq
Unknown
Sanger Sequencing - Human STAM (STAM1) knockout HEK-293T cell lysate (AB259158)
Allele-2 : 1 bp deletion in exon7
- Sanger seq
Unknown
Sanger Sequencing - Human STAM (STAM1) knockout HEK-293T cell lysate (AB259158)
Allele-1 : 20 bp deletion in exon7
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
STAM1 participates in endocytic trafficking and cytokine receptor endocytosis. It often associates with the ESCRT-0 (Endosomal Sorting Complex Required for Transport) complex which plays an essential role in sorting ubiquitinated cargo for lysosomal degradation. This protein helps regulate the cellular response to certain cytokines by facilitating the downregulation of activated receptors thereby contributing to signal attenuation.
Pathways
STAM1 involvement can be seen in cytokine signaling pathways and the ubiquitin-proteasome system. In the context of cytokine signaling STAM1 interacts with components like the Janus Kinase (JAK) family and Signal Transducers and Activators of Transcription (STATs) modulating cell growth and immune responses. It also has a significant function in the ubiquitin-dependent endocytic route coordinating with other ESCRT components like HRS (hepatocyte growth factor-regulated tyrosine kinase substrate).
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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