STAT1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon4 and 1 bp deletion in exon4 and 5 bp deletion in exon4.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon4 and 1 bp deletion in exon4 and 5 bp deletion in exon4.
Alternative names
CANDF7, DKFZp686B04100, IMD31A, IMD31B, IMD31C, ISGF-3, OTTHUMP00000163552, OTTHUMP00000165046, OTTHUMP00000165047, OTTHUMP00000205845, STAT1_HUMAN, STAT91, Signal transducer and activator of transcription 1, Signal transducer and activator of transcription 1 91kD, Signal transducer and activator of transcription 1 91kDa, Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF 3 components p91 p84, Transcription factor ISGF-3 components p91/p84, XStat1
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STAT1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon4 and 1 bp deletion in exon4 and 5 bp deletion in exon4.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon4 and 1 bp deletion in exon4 and 5 bp deletion in exon4.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- STAT1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Signal Transducer and Activator of Transcription 1 (STAT1) is a protein of about 91 kDa commonly known simply as STAT1. It belongs to the STAT protein family which is involved in gene regulation. STAT1 expresses widely in many tissues including the immune system where it plays a major role. As an important player in cellular responses to cytokines and growth factors STAT1 becomes activated through phosphorylation often referred to as phospho-STAT1. This phosphorylation promotes dimerization an important step for its function.
- Biological function summary
The STAT1 protein influences cell survival and immune responses. It functions as a transcription factor and participates in the interferon signaling pathway. In this setting it forms a complex with other STAT proteins such as STAT2 and STAT3 to regulate gene expression. By binding to specific DNA sequences in the nucleus STAT1 alters transcription in response to extracellular signals. It contributes to antiviral defense mechanisms and antiproliferative activities in various cells.
- Pathways
The STAT1 protein plays essential roles in the JAK-STAT signaling pathway and interferon signaling pathway. It operates closely with proteins such as JAK1 and TYK2 which are involved in the phosphorylation process activating STAT1. During this activation STAT1 helps initiate cellular responses to interferons a type of signaling protein released during viral infections. The JAK-STAT pathway facilitates various physiological processes including immune responses and cell growth regulation.
- Associated diseases and disorders
STAT1 dysregulation often connects to autoimmune diseases and infectious diseases. Elevated STAT1 activity associates with disorders like systemic lupus erythematosus where an imbalanced immune response occurs. Phosphorylation of STAT1 can also relate to chronic infections in cases where proper immune response activation becomes impaired. In certain cancers STAT1 interaction with proteins like SOCS1 might contribute to tumor immune evasion highlighting its vital role in pathology.
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5 product images
Western blot - Human STAT1 knockout HeLa cell lysate (ab263837)
Lane 1: Wild-type HeLa cell lysate 20 μg
Lane 2: STAT1 knockout HeLa cell lysate 20 μg
Lanes 1 - 2:Merged signal (red and green). Green - Anti-STAT1 alpha antibody [EPYR2154] ab92506 observed at 85 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
Anti-STAT1 alpha antibody [EPYR2154] ab92506 was shown to react with STAT1 alpha in wild-type HeLa cells in Western blot with loss of signal observed in STAT1 knockout cell line Human STAT1 knockout HeLa cell line ab255346 (STAT1 knockout cell lysate ab263837). Wild-type HeLa and STAT1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with Anti-STAT1 alpha antibody [EPYR2154] ab92506 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.All lanes: Western blot - Anti-STAT1 alpha antibody [EPYR2154] (Anti-STAT1 alpha antibody [EPYR2154] ab92506) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: STAT1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human STAT1 knockout HeLa cell line (Human STAT1 knockout HeLa cell line ab255346)
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 85 kDa
Western blot - Human STAT1 knockout HeLa cell lysate (ab263837)
Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: STAT1 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - Anti-STAT1 antibody [EPR4407] ab109320 observed at 87 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-STAT1 antibody [EPR4407] ab109320 Recombinant Anti-STAT1 antibody [EPR4407] was shown to specifically react with STAT1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human STAT1 knockout HeLa cell line ab255346 (knockout cell lysate ab263837) was used. Wild-type and STAT1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-STAT1 antibody [EPR4407] ab109320 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-STAT1 antibody [EPR4407] (Anti-STAT1 antibody [EPR4407] ab109320) at 1/10000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: STAT1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human STAT1 knockout HeLa cell line (Human STAT1 knockout HeLa cell line ab255346)
Performed under reducing conditions.
Predicted band size: 48 kDa, 87 kDa
Observed band size: 50 kDa, 87 kDa
Sanger Sequencing - Human STAT1 knockout HeLa cell lysate (ab263837)
Allele-2: 5 bp deletion in exon4
Sanger Sequencing - Human STAT1 knockout HeLa cell lysate (ab263837)
Allele-1: 14 bp deletion in exon4
Sanger Sequencing - Human STAT1 knockout HeLa cell lysate (ab263837)
Allele-3: 1 bp deletion in exon4
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