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STAT2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon2 and 1 bp deletion in exon2.

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Images

Western blot - Human STAT2 knockout A549 cell lysate (AB257185), expandable thumbnail
  • Sanger Sequencing - Human STAT2 knockout A549 cell lysate (AB257185), expandable thumbnail
  • Sanger Sequencing - Human STAT2 knockout A549 cell lysate (AB257185), expandable thumbnail

Key facts

Cell type
A549
Species or organism
Human
Tissue
Lung
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon2 and 1 bp deletion in exon2.

Alternative names

Homo sapiens interferon alpha induced transcriptional activator, ISGF-3, MGC59816, P113, STAT113, STAT2_HUMAN, Signal transducer and activator of transcription 2, interferon alpha induced transcriptional activator, signal transducer and activator of transcription 2 113kD

What's included?

1 Kit
Components
Human STAT2 knockout A549 cell lysate
1 x 100 µg
Human wild-type A549 cell lysate
1 x 100 µg

Recommended products

STAT2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon2 and 1 bp deletion in exon2.

Key facts

Cell type
A549
Mutation description
Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon2 and 1 bp deletion in exon2.
Disease
Carcinoma
Concentration
Loading...

Properties

Gene name
STAT2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Signal Transducer and Activator of Transcription 2 commonly known as STAT2 is a protein with a molecular mass of approximately 97 kDa. It functions mainly as a transcription factor and is an essential mediator of the interferon (IFN) signaling pathway. STAT2 is expressed in various tissues including immune cells where it responds to external stimuli. It works by translocating to the nucleus upon phosphorylation subsequently influencing the expression of genes involved in immune response.

Biological function summary

Members of the STAT protein family including STAT2 play a central role in mediating immune responses. STAT2 often forms a complex with STAT1 and other proteins such as IRF9 to initiate the transcription of interferon-stimulated genes (ISGs). This complex known as the ISGF3 complex facilitates the cellular response to viral infections by promoting the expression of antiviral proteins that help modify the host cellular environment to resist viral replication.

Pathways

The involvement of STAT2 is closely associated with the JAK-STAT signaling pathway and the interferon signaling pathway. In these pathways it interacts with proteins like JAK1 TYK2 and STAT1 playing a part in transmitting signals from the cell surface to the nucleus. Such pathways are fundamental to regulating the body's immune response to pathogens and managing cell growth and apoptosis in various cells.

Associated diseases and disorders

STAT2 alterations have been linked to autoimmune diseases and viral infections. Disruptions in STAT2 function can impair the body's ability to combat viruses effectively making individuals more susceptible to infections. STAT2 mutations or dysregulations might also connect to disorders like Systemic Lupus Erythematosus where immune system malfunctions occur. The interactions of STAT2 with other proteins like STAT1 and IRF9 in disease contexts underline its importance in maintaining immune system balance.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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