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AB257712

Human STK11 (LKB1) knockout HEK-293T cell lysate

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STK11 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

View Alternative Names

LKB1, Liver kinase B1, PJS, Polarization related protein LKB1, Renal carcinoma antigen NY-REN-19, STK11_HUMAN, Serine/Threonine Kinase 11, Serine/threonine protein kinase STK11, Serine/threonine-protein kinase 11, Serine/threonine-protein kinase LKB1, Serine/threonine-protein kinase XEEK1, hLKB1

2 Images
Western blot - Human STK11 (LKB1) knockout HEK-293T cell lysate (AB257712)
  • WB

Lab

Western blot - Human STK11 (LKB1) knockout HEK-293T cell lysate (AB257712)

Lane 1 : Wild-type HEK-293T cell lysate (20µg)

Lane 2 : STK11 knockout HEK-293T cell lysate (20µg)

Lane 3 : Hu testis cell lysate (20µg)

Lane 4 : HeLa cell lysate (20µg)

Lanes 1- 4 : Merged signal (red and green). Green - ab199970 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.

ab199970 Anti-LKB1 antibody [EPR19379] was shown to specifically react with LKB1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266647 (knockout cell lysate ab257712) was used. Wild-type and LKB1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab199970 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-LKB1 antibody [EPR19379] (<a href='/en-us/products/primary-antibodies/lkb1-antibody-epr19379-ab199970'>ab199970</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

STK11 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human STK11 (LKB1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-stk11-lkb1-knockout-hek-293t-cell-line-ab266647'>ab266647</a>)

Lane 3:

Hu testis cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Predicted band size: 48 kDa

Observed band size: 55 kDa

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Sanger Sequencing - Human STK11 (LKB1) knockout HEK-293T cell lysate (AB257712)
  • Sanger seq

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Sanger Sequencing - Human STK11 (LKB1) knockout HEK-293T cell lysate (AB257712)

Homozygous : 1 bp insertion in exon1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
STK11
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LKB1 also known as STK11 is a serine/threonine kinase with a molecular weight of approximately 48 kDa. This protein is widely expressed in various tissues including the liver pancreas and skeletal muscle. LKB1 plays an essential role in cellular energy homeostasis by phosphorylating and activating a group of at least 14 different proteins within the AMP-activated protein kinase (AMPK) family. The LKB1 protein acts as a master upstream kinase ensuring proper cellular energy balance and metabolism under conditions of nutrient stress.
Biological function summary

LKB1 is involved in controlling cell growth polarity and metabolism. It forms a complex with STRAD and MO25 which are necessary for its activation and function. By influencing these biological processes LKB1 regulates cell cycle arrest in response to stress signals and contributes to maintaining cellular integrity. The regulation of these processes positions LKB1 as an important factor in managing how cells respond to metabolic challenges.

Pathways

Its function integrates into major signaling networks like the AMPK and mTOR pathways. LKB1 activates AMPK which in turn affects mTOR signaling a pathway critical for cell growth and autophagy regulation. LKB1's interaction with AMPK links cellular energy levels to the control of biosynthetic pathways and cell cycle progression. The LKB1-AMPK-mTOR axis for instance highlights the protein's role in managing how cells balance growth-related activities and energy conservation.

LKB1 mutations or malfunctions are linked to Peutz-Jeghers syndrome and various forms of cancer such as lung cancer. Altered LKB1 activity affects its ability to suppress tumors primarily through its pathway interactions with AMPK and mTOR. Dysfunction in this pathway leads to unregulated cell growth and subsequently tumorigenesis. Understanding LKB1's role in these conditions aids in developing targeted therapies and diagnostic tools to manage associated risks and interventions effectively.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB199970

Anti-LKB1 antibody [EPR19379]

KO Validated|RabMAb|Recombinant

Immunoprecipitation - Anti-LKB1 antibody [EPR19379] (AB199970)

  • 5
  • 1
Cell Lines & Lysates

AB286626

Human STK11 knockout HCT116 cell line

Advanced Validation

Western blot - Human STK11 knockout HCT116 cell line (AB286626)

  • 0
  • 0
Primary Antibodies

AB40759

Anti-Smad4 antibody [EP618Y]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad4 antibody [EP618Y] (AB40759)

  • 4
  • 9
Primary Antibodies

AB62751

Anti-YAP1 (phospho Y357) antibody

Western blot - Anti-YAP1 (phospho Y357) antibody (AB62751)

  • 4
  • 1

Product promise

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