Human STK17A knockout HeLa cell lysate
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STK17A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.
View Alternative Names
DAP kinase-related apoptosis-inducing protein kinase 1, DRAK1, EC 2.7.11.1, ST17A_HUMAN, Serine / threonine kinase 17a death associated protein kinase related 1, Serine/threonine kinase 17a, Serine/threonine-protein kinase 17A, death-associated protein kinase-related 1
- Sanger seq
Unknown
Sanger Sequencing - Human STK17A knockout HeLa cell lysate (AB258705)
Allele-4 : Insertion of the selection cassette in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human STK17A knockout HeLa cell lysate (AB258705)
Allele-2 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human STK17A knockout HeLa cell lysate (AB258705)
Allele-1 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human STK17A knockout HeLa cell lysate (AB258705)
Allele-3 : Insertion of the selection cassette in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The kinase activity of STK17A affects processes like apoptosis and cell migration. It does not form a complex with other proteins but can directly interact with them to exert its influence. Through these interactions STK17A can regulate cell survival and death which are important in development and homeostasis within an organism.
Pathways
STK17A acts in the apoptosis signaling pathway and is known to modulate JNK (c-Jun N-terminal kinase) signaling. In the apoptosis pathway STK17A can modify the response of cells to stress stimuli effectively controlling programmed cell death. It shows functional association with proteins such as caspases which carry out the execution phase of apoptosis thereby linking STK17A to these essential cellular processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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