Human STK24 knockout A-431 cell lysate
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STK24 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 13 bp deletion, 24 bp deletion, 4 bp deletion; Frameshift = 76.79%.
View Alternative Names
HEL-S-95, MST-3, MST3/C, MST3/N, MST3B, Mammalian STE20-like protein kinase 3, Mammalian STE20-like protein kinase 3 C-terminal, Mammalian STE20-like protein kinase 3 N-terminal, Mammalian sterile 20-like 3, STE20 homolog yeast, STE20-like kinase MST3, STK24_HUMAN, Serine/threonine kinase 24 (Ste20, yeast homolog), Serine/threonine-protein kinase 24, Serine/threonine-protein kinase 24 12 kDa subunit, epididymis secretory protein Li 95, serine threonine kinase 24
- WB
Lab
Western blot - Human STK24 knockout A-431 cell lysate (AB269643)
Lane 1 : Wild-type A-431 cell lysate (20 ug)
Lane 2 : STK24 knockout A-431 cell lysate (20 ug)
Lane 3 : HeLa cell lysate (20 ug)
Lane 4 : Raji cell lysate (20 ug)
Lanes 1 - 4 : Merged signal (red and green). Green - ab51137 observed at 52 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab51137 was shown to react with MST3 in wild-type A431 cells in Western blot with loss of signal observed in STK24 knockout cell line ab269479 (knockout cell lysate ab269643). Wild-type A431 and STK24 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab51137 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-MST3 antibody [EP1468Y] (<a href='/en-us/products/primary-antibodies/mst3-antibody-ep1468y-ab51137'>ab51137</a>) at 1/1000 dilution
Lane 1:
Wild-type A431 cell lysate at 20 µg
Lane 2:
STK24 knockout A431 cell lysate at 20 µg
Lane 2:
Western blot - Human STK24 knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-stk24-knockout-a-431-cell-line-ab269479'>ab269479</a>)
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
Raji cell lysate at 20 µg
Predicted band size: 49 kDa
Observed band size: 52 kDa
false
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MST3 participates in regulating cell proliferation apoptosis and migration. It is not part of a larger complex but works closely with other signaling proteins to influence these processes. MST3 impacts cytoskeletal organization contributing to the dynamic remodeling of actin filaments. This protein also affects the apoptotic pathways by modulating the interactions with other kinases and apoptosis-related factors thereby aiding in the maintenance of cellular homeostasis.
Pathways
MST3 plays significant roles in the Hippo signaling pathway and the MAPK pathway—both critical for cell growth survival and differentiation. MST3 shares pathways with kinases like MOB1 and NDR1/2 in the Hippo pathway where it assists in regulating cell proliferation and apoptosis. In the context of MAPK signaling MST3 interacts with and is regulated by RAF1 and MEK1/2 coordinating cell stress responses and signaling cascades.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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