Human STK3 (MST-2) knockout HeLa cell lysate
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- WB
Lab
Western blot - Human STK3 (MST-2) knockout HeLa cell lysate (AB257714)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : STK3 knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab71960 observed at 56 kDa. Red - loading control, ab181602 observed at 37 kDa.
ab71960 Anti-STK3/MST-2 antibody [3067C3a] was shown to specifically react with STK3/MST-2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265082 (knockout cell lysate ab257714) was used. Wild-type and STK3/MST-2 knockout samples were subjected to SDS-PAGE. ab71960 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-STK3/MST-2 antibody [3067C3a] (<a href='/en-us/products/primary-antibodies/stk3-mst-2-antibody-3067c3a-ab71960'>ab71960</a>) at 1/10000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
STK3 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human STK3 (MST-2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-stk3-mst-2-knockout-hela-cell-line-ab265082'>ab265082</a>)
Predicted band size: 56 kDa
Observed band size: 56 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human STK3 (MST-2) knockout HeLa cell lysate (AB257714)
Homozygous : 1 bp insertion in exon2
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK3/MST-2 antibody [EP1466Y] (AB52641)](https://content.abcam.com/products/images/stk3-mst-2-antibody-ep1466y-ab52641--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img7026.jpg)
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
STK3/MST-2 is a core component of the Hippo signaling pathway which controls organ size by regulating cell proliferation and apoptosis. MST-2 often interacts with other proteins and participates in functional complexes notably with MOB1 and LATS1/2. These interactions are essential for transmitting growth inhibitory signals. Through its kinase activity MST-2 phosphorylates and activates these molecules initiating a cascade that ultimately influences gene expression impacting cell cycle and cell death processes.
Pathways
MST-2 plays a pivotal role not only in the Hippo pathway but also in the MAPK signaling network. In the Hippo pathway MST-2 phosphorylates components like LATS1/2 subsequently affecting the translocation and activity of YAP/TAZ transcription factors which control cell proliferation and survival. In the MAPK pathway MST-2 can interact with other kinases such as ASK1 to influence stress response signaling. These interactions demonstrate how MST-2 integrates signals between pathways to mediate critical decisions in cell life and death.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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