Human STK38L (Ndr2) knockout HeLa cell lysate
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STK38L KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 5 bp deletion in exon2 and Insertion of the selection cassette in exon2.
View Alternative Names
KIAA0965, NDR2, NDR2 protein kinase, Nuclear Dbf2-related kinase 2, ST38L_HUMAN, Serine/threonine-protein kinase 38-like, Stk38l, nuclear Dbf2 related 2
- Sanger seq
Unknown
Sanger Sequencing - Human STK38L (Ndr2) knockout HeLa cell lysate (AB259162)
Allele-2 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human STK38L (Ndr2) knockout HeLa cell lysate (AB259162)
Allele-1 : 5 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human STK38L (Ndr2) knockout HeLa cell lysate (AB259162)
Allele-3 : Insertion of the selection cassette in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
STK38L acts as an important regulator by coordinating cell cycle-related activities and influences apoptosis. It is known to participate as part of the larger signaling complex interacting with scaffold proteins to ensure proper cell function. The protein mediates these biological processes through phosphorylation impacting various signaling networks and helping cells respond to different physiological conditions effectively.
Pathways
STK38L integrates into key signaling pathways such as the Hippo pathway which is essential for controlling organ size through the regulation of cell proliferation and apoptosis. This pathway involves other proteins like MST1/2 and LATS1/2 which closely interact with STK38L to maintain cellular homeostasis. The interaction with these proteins illustrates STK38L's critical positioning in cellular communication networks affecting the pathway’s outcome on cellular behavior.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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