JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB259163

Human STRAP (Unrip) knockout HEK-293T cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

STRAP KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 3 and 1 bp insertion in exon 3 and 5 bp deletion in exon 3.

View Alternative Names

MAP activator with WD repeats, MAWD, PTWD, STRAP_HUMAN, Serine-threonine kinase receptor-associated protein, UNR-interacting protein, UNRIP, WD-40 repeat protein PT-WD, serine/threonine kinase receptor associated protein

3 Images
Sanger Sequencing - Human STRAP (Unrip) knockout HEK-293T cell lysate (AB259163)
  • Sanger seq

Unknown

Sanger Sequencing - Human STRAP (Unrip) knockout HEK-293T cell lysate (AB259163)

Allele-3 : 1 bp insertion in exon 3

Sanger Sequencing - Human STRAP (Unrip) knockout HEK-293T cell lysate (AB259163)
  • Sanger seq

Unknown

Sanger Sequencing - Human STRAP (Unrip) knockout HEK-293T cell lysate (AB259163)

Allele-1 : 14 bp deletion in exon 3

Sanger Sequencing - Human STRAP (Unrip) knockout HEK-293T cell lysate (AB259163)
  • Sanger seq

Unknown

Sanger Sequencing - Human STRAP (Unrip) knockout HEK-293T cell lysate (AB259163)

Allele-2 : 5 bp deletion in exon 3

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 3 and 1 bp insertion in exon 3 and 5 bp deletion in exon 3.

style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab259163-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human STRAP knockout HEK293T cell lysate", "number":"AB259163-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB259163-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
STRAP
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Unrip also known as NRIP (nuclear receptor interaction protein) is a nuclear protein with a molecular mass of approximately 64 kDa. Researchers have identified its expression mainly in the nucleus across various tissues. Mechanically Unrip functions as a factor interacting with nuclear hormone receptors. It regulates transcriptional activity through direct contact with these receptors modulating gene expression. This interaction plays a role in how cells respond to specific hormones and signals.
Biological function summary

Unrip contributes significantly to the regulation of gene expression by associating with other nuclear coregulators. It is part of a larger protein complex that includes importin beta and RIC1/RAB1. This complex facilitates the proper localization and functioning of nuclear hormone receptors affecting the expression of target genes. Unrip’s involvement with such complexes illustrates its integral function in transcriptional regulation and cellular signaling pathways.

Pathways

Unrip integrates into essential signaling networks particularly the nuclear receptor signaling and coactivator pathways. In these pathways Unrip modulates the activity of nuclear receptors like estrogen receptor alpha and glucocorticoid receptor affecting cellular responses to steroid hormones. Unrip interacts with proteins such as SRC-1 and GRIP1 within these pathways influencing gene expression profiles related to growth differentiation and metabolic processes.

Unrip’s interactions impact conditions including cancer and hormone-related disorders. Its role in modulating estrogen and glucocorticoid receptors links it to breast cancer progression where these pathways are dysregulated. Additionally Unrip’s involvement in nuclear receptor signaling ties it to metabolic disorders like type 2 diabetes. Through the alteration of receptor pathways Unrip affects proteins like PPARγ demonstrating its relevance in disease mechanisms and therapeutic research.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Complementary Products

Cell Lines & Lysates

AB266578

Human STRAP (Unrip) knockout HEK-293T cell line

Sanger Sequencing - Human STRAP (Unrip) knockout HEK-293T cell line (AB266578)

  • 0
  • 0
Primary Antibodies

AB205015

Anti-Unrip antibody

Immunoprecipitation - Anti-Unrip antibody (AB205015)

  • 0
  • 0
Primary Antibodies

AB32518

Anti-IKB alpha antibody [E130]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-IKB alpha antibody [E130] (AB32518)

  • 4
  • 31
Primary Antibodies

AB219413

Anti-MyD88 antibody [EPR21824]

RabMAb|Recombinant|KO Validated

Western blot - Anti-MyD88 antibody [EPR21824] (AB219413)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com