Human STT3B knockout HEK-293T cell lysate
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(1 Publication)
STT3B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 277 bp deletion in exon 1.
View Alternative Names
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B, Homolog of yeast STT3, Oligosaccharyl transferase subunit STT3B, SIMP, STT3 subunit of the oligosaccharyltransferase complex homolog B, STT3 subunit of the oligosaccharyltransferase complex homolog B (S. cerevisiae), STT3B_HUMAN, Source of immunodominant MHC-associated peptides homolog, source of immunodominant MHC associated peptides
- Sanger seq
Unknown
Sanger Sequencing - Human STT3B knockout HEK-293T cell lysate (AB259165)
Homozygous : 277 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Oligosaccharide transfer processes necessitate the presence of STT3B which acts as a catalytic subunit. STT3B is vital in the post-translational modification process as it participates in co-translational and post-translational N-linked glycosylation. As part of the Sec61 complex it collaborates with other subunits such as STT3A to ensure complete glycosylation particularly for those sites missed by STT3A during initial protein synthesis.
Pathways
Protein glycosylation heavily relies on STT3B's function. It integrates within the N-glycan biosynthesis pathway influencing protein quality control and degradation pathways. Alongside proteins like STT3A and other components of the oligosaccharyltransferase complex it determines proper protein localization and stability. This pathway contributes to cellular processes linked with protein maturation and trafficking.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Publications (1)
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International journal of oncology 58:122-132 PubMed33367935
2020
Applications
Unspecified application
Species
Unspecified reactive species
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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