Human STX10 knockout HEK-293T cell lysate
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STX10 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 25 bp deletion in exon 1.
View Alternative Names
SYN10, hsyn10, syntaxin 10
- Sanger seq
Unknown
Sanger Sequencing - Human STX10 knockout HEK-293T cell lysate (AB259166)
Homozygous : 25 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein Syntaxin 10 contributes to the trans-Golgi network and endosome system. It acts as part of the SNARE complex interacting with other SNARE proteins like Vti1a and VAMP3 to facilitate the tethering and fusion of transport vesicles. Through these interactions STX10 ensures the correct delivery of cargo to target membranes impacting processes like cell communication and maintenance of cellular structure.
Pathways
This protein Syntaxin 10 participates in the Golgi-to-endosome transport pathway and endosomal-lysosomal transport. In these pathways STX10 closely associates with proteins like STX16 and VTI1A which collaborate in endosomal cargo selection and vesicle formation. These interactions are essential for maintaining membrane dynamics and protein sorting supporting cellular logistics and waste disposal within cells.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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