Human SUCLG2 knockout HEK-293T cell lysate
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SUCLG2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.
View Alternative Names
EC 6.2.1.4, G BETA, GTP specific succinyl-CoA synthetase beta subunit, GTP-specific succinyl-CoA synthetase subunit beta, SCS-betaG, SUCB2_HUMAN, Succinate CoA ligase GDP forming beta subunit, Succinate-Coenzyme A ligase, GDP-forming, beta subunit, Succinyl CoA ligase GDP forming beta chain mitochondrial, Succinyl-CoA ligase [GDP-forming] subunit beta, Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial, Succinyl-CoA synthetase beta-G chain, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human SUCLG2 knockout HEK-293T cell lysate (AB259168)
Allele-1 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human SUCLG2 knockout HEK-293T cell lysate (AB259168)
Allele-2 : Insertion of the selection cassette in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SUCLG2 contributes to the production of GTP through substrate-level phosphorylation an important step in the tricarboxylic acid (TCA) cycle. As part of the succinyl-CoA synthetase complex it aids in the intricate process of energy conversion within mitochondria. The enzymatic activity of SUCLG2 ensures the proper balance of GTP within cells influencing various metabolic processes essential for cellular function and maintenance.
Pathways
SUCLG2 operates within the TCA cycle and is a pivotal link in cellular respiration. In this pathway it ensures the conversion of carbon and energy flow important for energy production. SUCLG2's role here is closely related to the protein SUCLG1 with which it forms the functional succinyl-CoA synthetase complex. Additionally SUCLG2 participates in metabolic pathways related to the biosynthesis of amino acids further extending its influence on cellular metabolism.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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