Human SUGP2 (SFRS14) knockout HeLa cell lysate
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SUGP2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 459 bp deletion in exon3.
View Alternative Names
Arginine/serine-rich-splicing factor 14, DKFZp779L2418, KIAA0365, SUGP2_HUMAN, SURP and G-patch domain-containing protein 2, Splicing factor, Splicing factor, arginine/serine-rich 14, arginine/serine-rich 14
- Sanger seq
Unknown
Sanger Sequencing - Human SUGP2 (SFRS14) knockout HeLa cell lysate (AB263375)
Homozygous : 459 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SFRS14 influences the regulation of alternative splicing by interacting with other splicing factors. It acts as part of the spliceosome complex which is responsible for the precise cutting and joining of exons from mRNA precursors. SFRS14 ensures the correct splicing of transcripts related to various cellular functions. Its role in alternative splicing helps generate protein diversity which is essential in maintaining cellular processes and adaptation to different physiological conditions.
Pathways
This protein takes part in the mRNA processing pathway and the gene expression pathway. In the mRNA processing pathway SFRS14 assists in accurately assembling the spliceosome ensuring proper splicing of precursor mRNA. Relating to other proteins in these pathways SFRS14 interacts with members of the SR protein family which includes proteins like SFRS1 and SFRS2. These interactions highlight its contribution to the regulation of gene expression through mRNA maturation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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