Human SUPT20H (p38IP) knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human SUPT20H (p38IP) knockout HEK-293T cell lysate (AB259169)
Homozygous : 323 bp deletion in exon6
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein plays a role in cellular signaling and stress responses. p38IP does not function alone; it is a component of the p38 MAPK signaling complex. By interacting with other members of this pathway such as MKK3 and MKK6 p38IP modulates cellular responses to external stressors. Its involvement in the signaling process highlights its contribution to the control of cellular dynamics under stress.
Pathways
P38IP holds significance in the MAPK signaling pathway and the JNK signaling pathway. In the MAPK signaling pathway it interfaces closely with p38 MAPK and other kinases facilitating signal transduction that impacts processes like inflammation and apoptosis. In the JNK signaling pathway interactions with JNK MAPKs suggest a role in regulating gene expression in response to stress stimuli. These pathways underline the regulatory capacity of p38IP in controlling cellular fate during stress conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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