SUPT20H KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 323 bp deletion in exon6.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 323 bp deletion in exon6.
Alternative names
AA667204, AI450544, C13, C13orf19, D3Ertd300e, FA48A_HUMAN, FAM48A, FP757, Protein FAM48A, RGD1307812, SPT20, bA421P11.4, p38-interacting protein, p38IP
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SUPT20H KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 323 bp deletion in exon6.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 323 bp deletion in exon6.
- Concentration
Properties
- Gene name
- SUPT20H
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
P38-interacting protein (p38IP) functions mechanically as a scaffold protein that interacts with p38 MAPK aiding in its regulation and stabilization. It is occasionally referred to as MO25. The molecular mass of p38IP is approximately 50 kDa. Expression of p38IP is observed in various tissues with notably high levels in the brain and the heart. This distribution suggests its involvement in processes across different cell types.
- Biological function summary
This protein plays a role in cellular signaling and stress responses. p38IP does not function alone; it is a component of the p38 MAPK signaling complex. By interacting with other members of this pathway such as MKK3 and MKK6 p38IP modulates cellular responses to external stressors. Its involvement in the signaling process highlights its contribution to the control of cellular dynamics under stress.
- Pathways
P38IP holds significance in the MAPK signaling pathway and the JNK signaling pathway. In the MAPK signaling pathway it interfaces closely with p38 MAPK and other kinases facilitating signal transduction that impacts processes like inflammation and apoptosis. In the JNK signaling pathway interactions with JNK MAPKs suggest a role in regulating gene expression in response to stress stimuli. These pathways underline the regulatory capacity of p38IP in controlling cellular fate during stress conditions.
- Associated diseases and disorders
P38IP associates with cancer progression and inflammatory disorders. Abnormal expression or malfunction of p38IP can contribute to dysregulated cell signaling in cancer partly through its relationship with p38 MAPK a known mediator of cell proliferation. In inflammatory disorders alterations in p38IP levels can affect immune response signaling also mediated through its partner JNK MAPKs illustrating its potential as a therapeutic target in these diseases.
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1 product image
Sanger Sequencing - Human SUPT20H (p38IP) knockout HEK-293T cell lysate (ab259169)
Homozygous: 323 bp deletion in exon6
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