Human SURF4 knockout HEK-293T cell lysate
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SURF4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 2.
View Alternative Names
1464:, AL033373, ERV 29, FLJ22993, MGC102753, MGC165910, MGC65806, MGC76061, SURF4_HUMAN, Surface 4 integral membrane protein, Surfeit 4, Surfeit locus protein 4, zgc:65806, zgc:77007
- Sanger seq
Unknown
Sanger Sequencing - Human SURF4 knockout HEK-293T cell lysate (AB259170)
Homozygous : 13 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The functions of SURF4 integrate into cellular processes through its involvement in protein secretion and sorting pathways. SURF4 participates as part of a complex that mediates the trafficking of glycoproteins which are essential for numerous cellular activities. The protein assists in the maintenance of quality control within the secretory pathway and ensures that proteins reach their correct destinations which is critical for proper cell function.
Pathways
SURF4 engages actively in processes related to protein transport and glycoprotein trafficking. It participates in the secretory pathway ensuring efficient delivery of proteins to the Golgi apparatus. The protein relates closely to other endoplasmic reticulum proteins like EMC1 which is involved in the coordinated movement and processing of proteins within the cell. SURF4's role intertwines with these components influencing cellular homeostasis and protein synthesis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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