SURF6 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1 and 7 bp deletion in exon1.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1 and 7 bp deletion in exon1.
Alternative names
SURF6_HUMAN, Surfeit locus protein 6
What's included?
Recommended products
SURF6 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1 and 7 bp deletion in exon1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1 and 7 bp deletion in exon1.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- SURF6
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Surf6 also known as SURF-6 or Surfeit locus protein 6 is a protein that acts mechanically within the cell nucleus. It is approximately 37 kDa in size and shows a nuclear localization. Surf6 expression takes place in a variety of cell types where it is often located in the nucleolus suggesting its role in ribosomal functions.
- Biological function summary
Within the nucleolus Surf6 associates with rRNA processing and ribosome biogenesis. It serves as a nucleolar protein with a function in facilitating the maturation and assembly of pre-rRNA into functional ribosomal subunits. Surf6 is not known to form part of a larger complex but shows interactions with other nucleolar components implying a regulatory role in ribosome production.
- Pathways
Elements involving Surf6 participate in the ribosome biogenesis pathway and the cell cycle regulation pathway. Surf6 has a documented association with the RNA polymerase I machinery which is important for rRNA transcription. Its function aligns with nucleolar proteins like nucleolin and fibrillarin which also regulate ribosomal RNA synthesis and processing.
- Associated diseases and disorders
Alterations in Surf6 expression or mutation can link to proliferative diseases such as cancer. Surf6's role in ribosome biogenesis ties it to tumors where excessive ribosome production supports uncontrolled cell division. Connections are evident with other proteins like p53 known for tumor suppressor activities highlighting Surf6's potential impact on cellular proliferation in oncogenic contexts.
Product promise
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Terms & Conditions.
2 product images
Sanger Sequencing - Human SURF6 knockout HeLa cell lysate (ab263376)
Allele-1: 7 bp deletion in exon1
Sanger Sequencing - Human SURF6 knockout HeLa cell lysate (ab263376)
Allele-2: 2 bp deletion in exon1
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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