Human SUZ12 knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
SUZ12 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 23 bp deletion in exon 1.
View Alternative Names
CHET9, ChET 9 protein, Chromatin precipitated E2F target 9 protein, JJAZ1, Joined to JAZF1 protein, KIAA0160, Polycomb protein SUZ12, SUZ12 polycomb repressive complex 2 subunit, SUZ12_HUMAN, Suppressor of zeste 12 homolog, Suppressor of zeste 12 protein homolog
- WB
Unknown
Western blot - Human SUZ12 knockout HeLa cell lysate (AB257721)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : SUZ12 knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab175187 observed at 100 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab175187 Anti-SUZ12 antibody [EPR5234(N)] - ChIP Grade was shown to specifically react with SUZ12 in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab264983 (knockout cell lysate ab257721) lane below 100kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and SUZ12 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab175187 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-SUZ12 antibody [EPR5234(N)] - ChIP Grade (<a href='/en-us/products/primary-antibodies/suz12-antibody-epr5234n-chip-grade-ab175187'>ab175187</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SUZ12 knockout HeLa cell lysate at 20 µg
Predicted band size: 83 kDa
Observed band size: 100 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human SUZ12 knockout HeLa cell lysate (AB257721)
Homozygous : 23 bp deletion in exon 1
- WB
Lab
Western blot - Human SUZ12 knockout HeLa cell lysate (AB257721)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : SUZ12 knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab12073 Anti-SUZ12 antibody - ChIP Grade was shown to specifically react with SUZ12 in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab264983 (knockout cell lysate ab257721) lane below 95kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and SUZ12 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab12073 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-SUZ12 antibody (<a href='/en-us/products/primary-antibodies/suz12-antibody-ab12073'>ab12073</a>) at 1 µg/mL
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SUZ12 knockout HeLa cell lysate (ab257721) at 20 µg
Predicted band size: 83 kDa
Observed band size: 95 kDa
false
Reactivity data
Product details
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB175187
Anti-SUZ12 antibody [EPR5234(N)] - ChIP Grade
RabMAb|Advanced Validation|Recombinant|KO Validated
![Immunocytochemistry/ Immunofluorescence - Anti-SUZ12 antibody [EPR5234(N)] - ChIP Grade (AB175187)](https://content.abcam.com/products/images/suz12-antibody-epr5234n-chip-grade-ab175187--immunocytochemistry-immunofluorescence-img1081.jpg)
- 1
- 1
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com