Human SYK knockout HEK-293T cell lysate

• WB

Lab

Western blot - Human SYK knockout HEK-293T cell lysate (AB283048)

Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : SYK knockout HEK-293T cell lysate 20 μg
Lane 3 : HAP1 cell lysate 20 μg
False colour image of Western blot : Anti-Syk antibody [SYK-01] staining at 1 μg/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab3993 was shown to bind specifically to Syk. A band was observed at 72/73 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SYK knockout cell line ab282649 (knockout cell lysate ab283048). To generate this image, wild-type and SYK knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-Syk antibody [SYK-01] (<a href='/en-us/products/primary-antibodies/syk-antibody-syk-01-ab3993'>ab3993</a>) at 1 µg/mL

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

SYK knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human SYK knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-syk-knockout-hek-293t-cell-line-ab282649'>ab282649</a>)

Lane 3:

HAP1 cell lysate at 20 µg

Predicted band size: 72 kDa

Observed band size: 72 kDa,73 kDa

false

• WB

Lab

Western blot - Human SYK knockout HEK-293T cell lysate (AB283048)

Lane 1 : Wild-type HEK-293T cell lysate, 20 ug

Lane 2 : SYK knockout HEK-293T cell lysate, 20 ug

False colour image of Western blot : Anti-Syk antibody [EP573Y] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab40781 was shown to bind specifically to Syk. A band was observed at 70/72 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SYK knockout cell line ab282649 (knockout cell lysate ab283048). To generate this image, wild-type and SYK knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

false

• Sanger seq

Supplier Data

Sanger Sequencing - Human SYK knockout HEK-293T cell lysate (AB283048)

127 bp deletion in exon 2