SYK KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 127 bp deletion in exon 2.
Images
Key facts
- Cell type
- HEK-293
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 127 bp deletion in exon 2
Alternative names
EC 2.7.10.2, KSYK_HUMAN, Spleen tyrosine kinase, Tyrosine-protein kinase SYK, kinase Syk, p72-Syk
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SYK KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 127 bp deletion in exon 2.
Key facts
- Cell type
- HEK-293
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 127 bp deletion in exon 2
- Concentration
Properties
- Gene name
- SYK
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Spleen tyrosine kinase commonly known as Syk is a non-receptor tyrosine kinase with a mass of about 72 kDa. It serves as a critical signaling molecule in immune cells. Syk is expressed in various cell types such as B cells T cells monocytes and others involved in the immune response. It initiates signaling cascades by binding to immunoreceptor tyrosine-based activation motifs (ITAMs). Alternate forms include phosphorylated Syk often referred to as p-Syk which activates various downstream signaling pathways.
- Biological function summary
Syk plays a role in transmitting signals from the cell surface to the interior of the cell facilitating various cellular activities. It is a part of signal transduction complexes and functions in the regulation of immune cell activation differentiation and survival. It affects processes like Fc receptor signaling in macrophages and neutrophils impacting immune responses and inflammation. The phospho-Syk form influences the strength and duration of signaling affecting various cellular functions.
- Pathways
Syk is significant in the B-cell receptor (BCR) signaling pathway and Fc receptor signaling pathway. Within these pathways Syk interacts with proteins like Lyn and Fyn which are other kinases involved in BCR signal transduction. Syk phosphorylation noted as p-Syk plays a part in activating downstream molecules such as phospholipase C gamma (PLCγ) to mediate cellular responses to external stimuli. The critical role of Syk in these pathways influences both adaptive and innate immune responses.
- Associated diseases and disorders
Syk correlates with conditions like rheumatoid arthritis and certain B-cell lymphomas. In rheumatoid arthritis aberrant Syk signaling contributes to the inflammation and joint destruction characteristic of the disease. Syk's role in B-cell antigen receptor signaling links it to B-cell lymphomas where dysregulation in signaling pathways leads to uncontrolled proliferation of malignant B cells. The involvement of Syk in both disease contexts suggests its potential as a therapeutic target along with pathways related to proteins like BLNK and PLCγ in these disorders.
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3 product images
Western blot - Human SYK knockout HEK-293T cell lysate (ab283048)
Lane 1: Wild-type HEK-293T cell lysate, 20 ugLane 2: SYK knockout HEK-293T cell lysate, 20 ug
False colour image of Western blot: Anti-Syk antibody [EP573Y] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Syk antibody [EP573Y] ab40781 was shown to bind specifically to Syk. A band was observed at 70/72 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SYK knockout cell line Human SYK knockout HEK-293T cell line ab282649 (knockout cell lysate ab283048). To generate this image, wild-type and SYK knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
Western blot - Human SYK knockout HEK-293T cell lysate (ab283048)
Lane 1: Wild-type HEK-293T cell lysate 20 μg
Lane 2: SYK knockout HEK-293T cell lysate 20 μg
Lane 3: HAP1 cell lysate 20 μg
False colour image of Western blot: Anti-Syk antibody [SYK-01] staining at 1 μg/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-Syk antibody [SYK-01] ab3993 was shown to bind specifically to Syk. A band was observed at 72/73 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SYK knockout cell line Human SYK knockout HEK-293T cell line ab282649 (knockout cell lysate ab283048). To generate this image, wild-type and SYK knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.All lanes: Western blot - Anti-Syk antibody [SYK-01] (Anti-Syk antibody [SYK-01] ab3993) at 1 µg/mL
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: SYK knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human SYK knockout HEK-293T cell line (Human SYK knockout HEK-293T cell line ab282649)
Lane 3: HAP1 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 72 kDa
Observed band size: 72 kDa, 73 kDa
Sanger Sequencing - Human SYK knockout HEK-293T cell lysate (ab283048)
127 bp deletion in exon 2
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