Human SYNJ1 (Synaptojanin) knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
- Sanger seq
Unknown
Sanger Sequencing - Human SYNJ1 (Synaptojanin) knockout HeLa cell lysate (AB257722)
Homozygous : 1 bp insertion in exon3
Complementary Products
![Immunohistochemistry (Frozen sections) - Anti-Synaptojanin 1 antibody [RP23040243] (AB308136)](https://content.abcam.com/products/images/synaptojanin-1-antibody-rp23040243-ab308136--immunohistochemistry-frozen-sections-img406969.jpg)
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Synaptojanin modulates synaptic function and plasticity through its role in lipid metabolism. It is part of a complex with endocytic proteins such as endophilin and dynamin which facilitates the uncoating of clathrin-coated vesicles following endocytosis. This complex action is important for maintaining synaptic efficiency and adaptability especially during high rates of synaptic activity. The disruption of synaptojanin's role affects the proper balance of phospholipids impacting neural communication and function.
Pathways
Synaptojanin plays a significant role in the phosphoinositide signaling and endocytic pathways. It works closely with other proteins like phosphatidylinositol-45-bisphosphate (PIP2) and adaptor protein 2 regulating membrane trafficking and the recycling of synaptic vesicles. These pathways are central to maintaining cellular homeostasis and ensuring neurotransmitter release and recycling occur effectively allowing cells to respond swiftly to dynamic signaling requirements.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com