Human SYNJ2 (Synaptojanin 2) knockout HeLa cell lysate
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SYNJ2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon3 and 68 bp deletion in exon3.
View Alternative Names
5-trisphosphate 5-phosphatase 2, INPP 5H, Inositol phosphate 5' phosphatase 2, KIAA0348, MGC44422, SJ2, SYNJ2_HUMAN, Synaptic inositol 1 4 5 trisphosphate 5 phosphatase 2, Synaptic inositol-1, Synaptojanin-2
- Sanger seq
Unknown
Sanger Sequencing - Human SYNJ2 (Synaptojanin 2) knockout HeLa cell lysate (AB258219)
Allele-2 : 68 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human SYNJ2 (Synaptojanin 2) knockout HeLa cell lysate (AB258219)
Allele-1 : 14 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Synaptojanin 2 plays an important role in synaptic vesicle trafficking and endocytosis. It can function as a part of protein complexes involved in these processes. The protein is involved in rearranging the actin cytoskeleton which impacts cellular shape and movement. Its activity influences synaptic transmission efficiency by controlling the cycle of synaptic vesicles particularly in the nervous system which reflects its high expression in the brain.
Pathways
Synaptojanin 2 is closely linked to the phosphatidylinositol signaling pathway and the endocytosis pathway. These pathways are important for cellular communication and nutrient uptake. Within these pathways synaptojanin 2 interacts with proteins like dynamin a GTPase that mediates membrane fission during endocytosis. Synaptojanin 2 also functions in relation to AP-2 a clathrin adaptor complex that plays a role in vesicle formation highlighting its participation in endocytic trafficking.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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