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AB257060

Human SYP (Synaptophysin) knockout HEK-293T cell lysate

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SYP KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 8 bp deletion in exon2.
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Western blot - Human SYP (Synaptophysin) knockout HEK-293T cell lysate (AB257060)
  • WB

Unknown

Western blot - Human SYP (Synaptophysin) knockout HEK-293T cell lysate (AB257060)

Lane 1 : Wild-type HEK293T cell lysate (20 μg)

Lane 2 : Syp knockout HEK293T cell lysate (20 μg)

Lane 3 : Human brain tissue lysate (20 μg)

Lanes 1-3 : Merged signal (red and green). Green - ab32127 observed at 38 kDa. Red - loading control, ab7291 observed at 50 kDa.

ab32127 Anti-Synaptophysin antibody [YE269] was shown to specifically react with Synaptophysin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267272 (knockout cell lysate ab257060) was used. Wild-type and Synaptophysin knockout samples were subjected to SDS-PAGE. ab32127 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti- Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Synaptophysin antibody [YE269] - Synaptic Marker (<a href='/en-us/products/primary-antibodies/synaptophysin-antibody-ye269-synaptic-marker-ab32127'>ab32127</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

Syp knockout HEK293T cell lysate at 20 µg

Lane 3:

Human brain tissue lysate at 20 µg

Predicted band size: 33 kDa

Observed band size: 38 kDa

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Western blot - Human SYP (Synaptophysin) knockout HEK-293T cell lysate (AB257060)
  • WB

Unknown

Western blot - Human SYP (Synaptophysin) knockout HEK-293T cell lysate (AB257060)

Lane 1 : Wild-type HEK293T cell lysate (20 μg)

Lane 2 : Syp knockout HEK293T cell lysate (20 μg)

Lane 3 : Human brain tissue lysate (20 μg)

Lanes 1-3 : Merged signal (red and green). Green - ab52636 observed at 38 kDa. Red - loading control, ab7291 observed at 50 kDa.

ab52636 Anti-Synaptophysin antibody [EP1098Y] was shown to specifically react with Synaptophysin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267272 (knockout cell lysate ab257060) was used. Wild-type and Synaptophysin knockout samples were subjected to SDS-PAGE. ab52636 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti- Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Synaptophysin antibody [EP1098Y] - Synaptic Marker (<a href='/en-us/products/primary-antibodies/synaptophysin-antibody-ep1098y-synaptic-marker-ab52636'>ab52636</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

Syp knockout HEK293T cell lysate at 20 µg

Lane 3:

Human brain tissue lysate at 20 µg

Predicted band size: 33 kDa

Observed band size: 38 kDa

false

Sanger Sequencing - Human SYP (Synaptophysin) knockout HEK-293T cell lysate (AB257060)
  • Sanger seq

Unknown

Sanger Sequencing - Human SYP (Synaptophysin) knockout HEK-293T cell lysate (AB257060)

Homozygous : 8 bp deletion in exon2

Western blot - Human SYP (Synaptophysin) knockout HEK-293T cell lysate (AB257060)
  • WB

Supplier Data

Western blot - Human SYP (Synaptophysin) knockout HEK-293T cell lysate (AB257060)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Performed under reducing conditions. In Western blot, ab309493 was shown to bind specifically to Synaptophysin (SYP).  Target of interest was observed at 38 kDa wild-type 293T cell lysates (lane 1) with no signal observed at this size in SYP knockout cell line (lane 2, knockout cell line ab267272 / knockout cell lysate ab257060). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 59 seconds.

All lanes:

Western blot - Anti-Synaptophysin antibody [YE269] - Synaptic marker - Mouse IgG1 (Chimeric) (<a href='/en-us/products/primary-antibodies/synaptophysin-antibody-ye269-synaptic-marker-mouse-igg1-chimeric-ab309493'>ab309493</a>) at 1/1000 dilution

Lane 1:

Wild-type 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

Western blot - Human SYP (Synaptophysin) knockout HEK-293T cell lysate (ab257060) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-fc-hrp-preadsorbed-ab98717'>ab98717</a>) at 1/20000 dilution

Observed band size: 38 kDa

false

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 8 bp deletion in exon2.

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Complementary Products

Primary Antibodies

AB32127

Anti-Synaptophysin antibody [YE269] - Synaptic Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB32127)

  • 5
  • 37
Primary Antibodies

AB184451

Anti-Dopamine Transporter antibody [EPR19695]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine Transporter antibody [EPR19695] (AB184451)

  • 0
  • 0
Primary Antibodies

AB18258

Anti-PSD95 antibody - Synaptic Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PSD95 antibody - Synaptic Marker (AB18258)

  • 5
  • 40
Primary Antibodies

AB254349

Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker

BOND RX™ Validated|Lab Essentials|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB254349)

  • 4
  • 1
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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
SYP
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Synaptophysin also known as SYP is a glycoprotein approximately 38 kDa in size. It is expressed abundantly in the presynaptic vesicle membranes of neurons and in neuroendocrine cells. Synaptophysin serves as a marker for synaptic vesicles and neuroendocrine tumors. The protein plays a significant role in synaptic vesicle cycling processes engaging in the release and recycling of neurotransmitters.
Biological function summary

Synaptophysin contributes to synaptic transmission and is engaged in the formation and maintenance of the synaptic vesicle pools. It is a part of the complex involved in neuronal communication and might interact with other vesicular proteins to ensure efficient neurotransmitter exchange. Researchers often utilize anti-Synaptophysin antibodies such as Alexa Fluor 647 labeled ones for visualization under confocal microscopy.

Pathways

Synaptophysin operates within pathways involving neurotransmitter release and synaptic vesicle cycle. It connects to proteins like Synaptotagmin and SNAP-25 integral to the facilitation of synaptic transmission and exocytosis. These pathways ensure efficient signal conduction across neurons which is critical for normal nervous system functioning.

Synaptophysin shows a strong association with neurodegenerative diseases and certain cancers like neuroblastoma. Altered expression levels or dysfunction of Synaptophysin can serve as a diagnostic marker for these conditions. The protein is connected with proteins like Bcl-2 in neurodegenerative diseases affecting cell survival and apoptosis pathways.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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