Human TAB1 knockout HEK-293T cell lysate
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TAB1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon2.
View Alternative Names
2310012M03Rik, 3'-Tab1, MAP3K7IP 1, MGC57664, Mitogen-activated protein kinase kinase kinase 7-interacting protein 1, TAB1_HUMAN, TAK1-binding protein 1, TGF-beta activated kinase 1/MAP3K7 binding protein 1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, TGF-beta-activated kinase 1-binding protein 1, Transforming growth factor beta activated kinase binding protein 1
- WB
Lab
Western blot - Human TAB1 knockout HEK-293T cell lysate (AB258220)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : TAB1 knockout HEK-293T cell lysate 20 μg
Lane 3 : U-2 OS cell lysate 20 μg
Lane 4 : HeLa cell lysate 20 μg
False colour image of Western blot : Anti-TAB1 antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab151408 was shown to bind specifically to TAB1. A band was observed at 60 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TAB1 knockout cell line ab266845 (knockout cell lysate ab258220). To generate this image, wild-type and TAB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 �C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution. 60 kDa observed band.
All lanes:
Western blot - Anti-TAB1 antibody (<a href='/en-us/products/primary-antibodies/tab1-antibody-ab151408'>ab151408</a>) at 1/500 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
TAB1 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human TAB1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-tab1-knockout-hek-293t-cell-line-ab266845'>ab266845</a>)
Lane 3:
U-2 OS cell lysate at 20 µg
Lane 4:
HeLa cell lysate at 20 µg
Predicted band size: 55 kDa
Observed band size: 60 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human TAB1 knockout HEK-293T cell lysate (AB258220)
Homozygous : 20 bp deletion in exon2
- WB
Supplier Data
Western blot - Human TAB1 knockout HEK-293T cell lysate (AB258220)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Performed under reducing conditions.
In Western blot, ab324014 was shown to bind specifically to TAB1. Target of interest was observed at 55 kDa in wild-type HEK293T cell lysate (Lane 1) with no signal observed at this size in TAB1 knockout cell line (Lane 2) (Lane 2, knockout cell line ab266845/ knockout cell lysate ab258220).
To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-TAB1 antibody [EPR29000-94] (<a href='/en-us/products/primary-antibodies/tab1-antibody-epr29000-94-ab324014'>ab324014</a>) at 1/1000 dilution
Lane 1:
Wild-type 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2:
Western blot - Human TAB1 knockout HEK-293T cell lysate (ab258220) at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 55 kDa,36 kDa
false
Exposure time: 92s
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TAB1 functions as a scaffold protein assisting in the assembly of signaling complexes. This protein is part of the TAK1 complex which also includes TAB2 and TAB3. The formation of this complex is essential for activating multiple intracellular signaling pathways. Through its role in the TAK1 complex TAB1 contributes to cellular responses to environmental cues including inflammation apoptosis and immune responses. Its activity influences transcription factor activation and is pivotal in orchestrating cellular adaptation to changes.
Pathways
TAB1 is essential in both the NF-kB and MAPK signaling pathways. Maintaining proper function in these pathways is important for inflammatory responses and stress-related signal transduction. TAB1 helps bridge the activation of TAK1 to the downstream signaling required for NF-kB pathway activation. Additionally in the MAPK pathway TAB1 influences the activation of proteins such as MKKs which further regulate cellular stress responses and developmental processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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