Human TACC1 knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
- Sanger seq
Unknown
Sanger Sequencing - Human TACC1 knockout HEK-293T cell lysate (AB259171)
Allele-1 : 19 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human TACC1 knockout HEK-293T cell lysate (AB259171)
Allele-2 : 8 bp deletion in exon3
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TACC1 engages in processes that contribute to proper cell division and genomic stability. It forms part of a larger molecular complex that includes components like the ch-TOG partner assisting in anchoring and stabilizing microtubules important for mitotic spindle function. TACC1 indirectly affects the balance between cell proliferation and apoptosis showing its importance in maintaining healthy cell cycles. Its role in spindle formation ensures accurate segregation of chromosomes which is critical for preventing aneuploidies.
Pathways
TACC1 is involved in mitotic signaling and microtubule organization pathways. It interacts with Aurora A kinase an essential element of the mitotic entry pathway ensuring that kinetochores are properly attached to spindle microtubules during mitosis. Another pathway involves the PI3K/AKT signaling where TACC1 may influence cellular responses to growth factors. Its interaction with Aurora A demonstrates the protein's importance in centrosome maturation and spindle stability highlighting its role in cell cycle regulation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com