Human TACC3 knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human TACC3 knockout HEK-293T cell lysate (AB257723)
Homozygous : Insertion of the selection cassette in exon 4
Complementary Products
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Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TACC3 is part of a protein complex that contributes to mitosis. It collaborates with the Aurora A kinase and ch-TOG protein to ensure proper microtubule dynamics and spindle formation. This complex stabilizes kinetochore microtubules by connecting them to the centrosomes allowing accurate chromosome segregation during cell division. TACC3's actions are fundamental to cell cycle regulation and maintaining genomic stability.
Pathways
The role of TACC3 is linked to the cell cycle regulation and mitosis pathway. It interacts closely with the Aurora A kinase pathway playing a role in regulating centrosome maturation and spindle assembly. In these pathways proteins like TPX2 and PLK1 are also involved working alongside TACC3 to maintain the fidelity of cell division and ensure equal distribution of genetic material to daughter cells.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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