Human TAGLN2 knockout HEK-293T cell lysate
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TAGLN2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2.
View Alternative Names
CDABP0035, HA1756, KIAA0120, SM22-alpha homolog, TAGL2_HUMAN, Transgelin-2, epididymis tissue protein Li 7e
- Sanger seq
Unknown
Sanger Sequencing - Human TAGLN2 knockout HEK-293T cell lysate (AB258707)
Allele-1 : 1 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human TAGLN2 knockout HEK-293T cell lysate (AB258707)
Allele-2 : 1 bp insertion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein aids in maintaining cell structure and facilitates cell movement and adhesion. TAGLN2 is a monomer and does not form complexes but interacts closely with actin to regulate cytoskeletal processes. It acts as a modulator of actin filament organization which impacts cellular responses. The protein supports processes such as wound healing and embryonic development by influencing the migration and invasion of cells.
Pathways
TAGLN2 influences cellular mechanisms related to the actin cytoskeleton structure and dynamics. It plays a role in the Rho signaling pathway which is essential for cytoskeletal rearrangement and maintaining cellular tension. The protein associates with RhoA a small GTPase which is involved in mediating various functions including contractility and motility. Through RhoA TAGLN2 impacts cell morphology and stress fiber formation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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