Human TAL1 knockout Jurkat cell lysate
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- NGS
Supplier Data
Next Generation Sequencing - Human TAL1 knockout Jurkat cell lysate (AB273816)
Knockout achieved by CRISPR/Cas9; X = 2 bp insertion; Frameshift : 100%
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The Tal1 protein controls the differentiation and proliferation of hematopoietic stem cells and progenitor cells. It participates as a part of a multiprotein complex involving other transcription factors and cofactors such as GATA-1 and LMO2 which modulates gene expression during blood cell lineage commitment. Through these interactions Tal1 influences both myeloid and lymphoid lineage differentiation.
Pathways
Tal1 acts as an important regulator in the hematopoietic development pathway and is critical for the endothelial-to-hematopoietic transition. This transition is supported by interaction with the E2A proteins which stabilize the formation of the transcription complex needed for lineage-specific gene regulation. Tal1 also plays a role in the VEGF signaling pathway impacting vascular and blood development processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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