Human TAOK2 knockout HEK-293T cell lysate
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TAOK2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.
View Alternative Names
1110033K02Rik, B230344N16, KIAA0881, Kinase from chicken homolog C, MAP3K17, PSK, PSK-1, PSK1 beta, Prostate derived STE20 like kinase PSK, Prostate derived sterile 20 like kinase 1, Prostate-derived STE20-like kinase 1, Serine/threonine-protein kinase TAO2, TAO 1, TAO 2, TAO kinase 2, TAOK2_HUMAN, Thousand and one amino acid protein 2, Thousand and one amino acid protein kinase, UNQ2971/PRO7431, hKFC-C, mKIAA0881
- Sanger seq
Unknown
Sanger Sequencing - Human TAOK2 knockout HEK-293T cell lysate (AB258709)
Homozygous : 1 bp insertion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TAOK2 is instrumental in modulating cytoskeletal dynamics impacting processes such as neuron morphology and dendritic branching. TAOK2 associates with complexes involved in microtubule assembly influencing cell division and intracellular transport. Its activity affects neuronal connectivity impacting neural development and synaptic function which are critical in the nervous system. This makes its regulation necessary for normal brain function and development.
Pathways
TAOK2 integrates into the MAPK signaling pathway affecting cell responses to stress and cytokines. This pathway is important for initiating cellular responses to external stimuli such as changes in the environment or cellular damage. TAOK2 phosphorylates other proteins such as MEK3 MEK6 and p38 MAP kinases influencing downstream signaling cascades. It also interacts with proteins involved in the Wnt signaling pathway contributing to cell differentiation proliferation and the maintenance of stem cells.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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