Human TAX1BP1 (TRAF6BP) knockout HEK-293T cell lysate
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TAX1BP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 5.
View Alternative Names
1200003J11Rik, 1700069J21Rik, AA930106, Aa1076, CALCOCO 3, D6Ertd404e, D6Ertd772e, Human T cell leukemia virus type 1-binding protein, Liver regeneration-related protein LRRG004, MGC94031, PRO0105, T6BP, TAX1BP1, TAX1BP1 protein, TAXB1_HUMAN, TRAF6 binding protein T6BP, TRAF6-binding protein, TRAF6-interacting protein, TXBP 151, Tax1 (human T cell leukemia virus type I) binding protein, Tax1 (human T cell leukemia virus type I) binding protein 1, Tax1 binding protein, Tax1-binding protein 1, Tax1-binding protein 1 homolog, tax1bp1b, wu:fc20c12, wu:fc56e11, zgc:73219, zgc:77129
- WB
Lab
Western blot - Human TAX1BP1 (TRAF6BP) knockout HEK-293T cell lysate (AB257726)
Anti-TAX1BP1 antibody [EPR13287(B)] (ab176572) staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab176572 was shown to bind specifically to TAX1BP1. A band was observed at 95 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TAX1BP1 knockout cell line. To generate this image, wild-type and TAX1BP1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes:
Western blot - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (<a href='/en-us/products/primary-antibodies/traf6bp-tax1bp1-antibody-epr13287b-c-terminal-ab176572'>ab176572</a>) at 1/2000 dilution
Lanes 1 - 2:
Western blot at 20 µg
Lane 2:
Western blot - Human TAX1BP1 (TRAF6BP) knockout HEK-293T cell lysate (ab257726)
Secondary
All lanes:
Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Predicted band size: 91 kDa
Observed band size: 95 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human TAX1BP1 (TRAF6BP) knockout HEK-293T cell lysate (AB257726)
Homozygous : 13 bp deletion in exon 5
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TRAF6BP/TAX1BP1 regulates the immune response through its interaction with TRAF6. It plays a part in the formation of a protein complex that influences the balance between pro-inflammatory and anti-inflammatory signals. TRAF6BP affects autophagy an important process for cellular homeostasis and pathogen elimination. TRAF6BP also manages cell survival and apoptosis through its role in signaling cascades.
Pathways
TRAF6BP/TAX1BP1 is heavily involved in the NF-kB and JNK signaling pathways which are pivotal for immune and inflammatory responses. TRAF6BP interacts with proteins like TRAF6 and TAK1 within these pathways to mediate downstream effects that lead to cytokine production and stress responses. These pathways play large roles in maintaining the immune system's balance.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB176572
Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal
RabMAb|Recombinant|KO Validated
![Western blot - Anti-TRAF6BP/TAX1BP1 antibody [EPR13287(B)] - C-terminal (AB176572)](https://content.abcam.com/products/images/traf6bp-tax1bp1-antibody-epr13287b-c-terminal-ab176572--western-blot-img116042.jpg)
- 0
- 0
![Western blot - Anti-ARPC2 antibody [EPR8533] (AB133315)](https://content.abcam.com/products/images/arpc2-antibody-epr8533-ab133315--western-blot-img32639.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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