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AB259175

Human TBKBP1 knockout HeLa cell lysate

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Human TBKBP1 knockout HeLa cell lysate. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

View Alternative Names

KIAA0775, ProSAPiP2, SINTBAD, TANK-binding kinase 1-binding protein 1, TBK1-binding protein 1, TBKB1_HUMAN

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Sanger Sequencing - Human TBKBP1 knockout HeLa cell lysate (AB259175)
  • Sanger seq

Unknown

Sanger Sequencing - Human TBKBP1 knockout HeLa cell lysate (AB259175)

Allele-1 : 1 bp insertion in exon6

Sanger Sequencing - Human TBKBP1 knockout HeLa cell lysate (AB259175)
  • Sanger seq

Unknown

Sanger Sequencing - Human TBKBP1 knockout HeLa cell lysate (AB259175)

Allele-2 : 4 bp insertion in exon6

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6 and 4 bp insertion in exon6.

Disease

Adenocarcinoma

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
TBKBP1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TBKBP1 also known as TANK-Binding Kinase 1 Binding Protein 1 is a protein involved in several cellular processes. It has a mass of around 61 kDa. TBKBP1 is widely expressed in various tissues including the heart brain liver and kidney. Mechanically TBKBP1 interacts with TBK1 (TANK-binding kinase 1) influencing signal transduction and the regulation of innate immune responses.
Biological function summary

TBKBP1 functions as a modulator of immune signaling pathways. It often associates with complexes involving adaptor proteins such as TANK and IKK epsilon (IKKε). TBKBP1 plays a significant role in regulating antiviral responses by facilitating the activation of transcription factors that induce the expression of interferon-stimulated genes. These activities highlight its involvement in the regulation of the immune system’s response to viral infections.

Pathways

TBKBP1 participates in the type I interferon signaling pathway and NF-kB signaling pathway. Within these pathways it works closely with proteins like TBK1 and IKKε contributing to cellular responses against viral and inflammatory stimuli. Through these interactions TBKBP1 helps modulate the production of cytokines and other mediators of inflammation.

TBKBP1 has connections to neurodegenerative diseases and cancer. Alterations in its normal function can result in impaired immune responses potentially contributing to the development of conditions like amyotrophic lateral sclerosis (ALS) and various forms of cancer. The interaction between TBKBP1 and TBK1 links it to pathological processes involving aberrant immune signaling and cellular stress responses.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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