Human TBRG4 knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human TBRG4 knockout HEK-293T cell lysate (AB263381)
Homozygous : 1 bp insertion in exon 2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TBRG4 influences several cellular processes through its involvement in protein interactions. It is an integral part of large molecular complexes that regulate various gene expression and remodeling of chromatin. TBRG4 often functions in concert with other components to ensure accurate modulation of transcriptional activity. Complex formation affects both direct regulation of target genes and the structural rearrangements necessary for cellular homeostasis.
Pathways
The protein plays a role in the cellular response to stress and metabolic signaling. It acts within the mTOR signaling pathway which is central to the regulation of growth and metabolism. TBRG4 interacts with other proteins like Raptor and mLST8 to modulate mTOR complex functions. Additionally it is related to the PKC signaling pathway where it may indirectly influence cellular responses to external stimuli through modulation of other participating proteins.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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