Human TCF7 knockout HeLa cell lysate

• WB

Lab

Western blot - Human TCF7 knockout HeLa cell lysate (AB258225)

Lane 1 : Wild-type Hela cell lysate 20 μg

Lane 2 : TCF7 knockout HeLa cell lysate 20 μg

Lane 3 : Jurkat cell lysate 20 μg

Lane 4 : HT29 cell lysate 20 μg

Lanes 1 - 4 : Merged signal (red and green). Green - anti-TCF7 antibody observed at 48 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

anti-TCF7 antibody was shown to react with TCF7 in wild-type HeLa cells in Western blot with loss of signal observed in TCF7 knockout cell line ab265127 (TCF7 knockout cell lysate 258225). Wild-type HeLa and TCF7 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with anti-TCF7 antibody and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and 1/20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 h at room temperature before imaging.

All lanes:

anti-TCF7 antibody at 1/1000 dilution

Lane 1:

Wild-type Hela cell lysate at 20 µg

Lane 2:

TCF7 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human TCF7 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-tcf7-knockout-hela-cell-line-ab265127'>ab265127</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HT29 cell lysate at 20 µg

false

• Sanger seq

Unknown

Sanger Sequencing - Human TCF7 knockout HeLa cell lysate (AB258225)

Allele-2 : 1 bp deletion in exon4

• Sanger seq

Unknown

Sanger Sequencing - Human TCF7 knockout HeLa cell lysate (AB258225)

Allele-3 : 1 bp insertion in exon4

• Sanger seq

Unknown

Sanger Sequencing - Human TCF7 knockout HeLa cell lysate (AB258225)

Allele-1 : 7 bp deletion in exon4