Human TDP2 knockout HeLa cell lysate
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TDP2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1 and Insertion of the selection cassette in exon1.
View Alternative Names
5'-Tyr-DNA phosphodiesterase, 5'-tyrosyl-DNA phosphodiesterase, AD 022, EAP 2, EAP II, ETS1-associated protein 2, ETS1-associated protein II, MGC111021, MGC9099, RP1-30M3.3, TRAF and TNF receptor-associated protein, TTRAP, TYDP2_HUMAN, Tyr-DNA phosphodiesterase 2, Tyrosyl-DNA phosphodiesterase 2, dJ30M3.3
- Sanger seq
Unknown
Sanger Sequencing - Human TDP2 knockout HeLa cell lysate (AB263382)
Allele-2 : Insertion of the selection cassette in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human TDP2 knockout HeLa cell lysate (AB263382)
Allele-1 : 2 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TDP2 plays an important role in DNA repair mechanisms. It participates in excising 5'-phosphotyrosyl moieties linked to DNAn important for maintaining genomic stability. TDP2 does not work as a part of a large protein complex but its activity supports several cellular processes by ensuring proper DNA repair. It facilitates the release of covalent protein-DNA complexes therefore aiding cell survival and function.
Pathways
TDP2 contributes to the DNA damage response and repair pathways. It acts in concert with proteins like XRCC1 and PARP1 to ensure repair of DNA strand breaks. Additionally TDP2 is involved in the non-homologous end joining (NHEJ) pathway an important mechanism for repairing double-strand breaks. Its function complements topoisomerase-related activities maintaining genomic integrity during cell division.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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