Human TDRD7 knockout A549 cell lysate
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TDRD7 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon8 and 2 bp deletion in exon8 and 2 bp insertion in exon8.
View Alternative Names
CATC4, KIAA1529, PCTAIRE2 BP, PCTAIRE2-binding protein, RP11-508D10.1, TDRD7_HUMAN, TR AP, Tudor domain containing 7, Tudor domain-containing protein 7, Tudor repeat associator with PCTAIRE 2
- Sanger seq
Unknown
Sanger Sequencing - Human TDRD7 knockout A549 cell lysate (AB258227)
Allele-1 : 2 bp deletion in exon8
- Sanger seq
Unknown
Sanger Sequencing - Human TDRD7 knockout A549 cell lysate (AB258227)
Allele-2 : 1 bp deletion in exon8
- Sanger seq
Unknown
Sanger Sequencing - Human TDRD7 knockout A549 cell lysate (AB258227)
Allele-3 : 2 bp insertion in exon8
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TDRD7 contributes to the formation and function of ribonucleoprotein (RNP) complexes needed for germ cell development and lens maturation. It actively participates in RNA granule assembly important for RNA stabilization and localization in cells. Through its Tudor domains TDRD7 binds symmetrically dimethylated arginines on other proteins recognizing and organizing these RNP complexes effectively.
Pathways
TDRD7 integrates into RNA regulatory pathways influencing processes such as mRNA splicing and degradation. It interacts with proteins like PIWIL1 and PIWIL2 which are involved in the piRNA pathway important for maintaining genomic integrity in germ cells. TDRD7 helps mediate gene expression regulation through these pathways impacting cellular functions and organismal development.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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