JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB258715

Human TECR (GPSN2) knockout HEK-293T cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

TECR KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1.
1 Images
Sanger Sequencing - Human TECR (GPSN2) knockout HEK-293T cell lysate (AB258715)
  • Sanger seq

Unknown

Sanger Sequencing - Human TECR (GPSN2) knockout HEK-293T cell lysate (AB258715)

Homozygous : 2 bp deletion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1.

style
left-column

Complementary Products

Primary Antibodies

AB247124

Anti-GPSN2 antibody

Western blot - Anti-GPSN2 antibody (AB247124)

  • 0
  • 0
Cell Lines & Lysates

AB266276

Human TECR (GPSN2) knockout HEK-293T cell line

Sanger Sequencing - Human TECR (GPSN2) knockout HEK-293T cell line (AB266276)

  • 0
  • 0
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258715-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human TECR knockout HEK293T cell lysate", "number":"AB258715-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB258715-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
TECR
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GPSN2 protein also known as Glycoprotein Subunit N2 plays an important role in the regulation of cellular processes. It has an approximate molecular mass of 45 kDa. GPSN2 is expressed in various tissues including the liver kidney and heart reflecting its involvement in different physiological contexts. This protein is involved in the post-translational modification of proteins specifically in N-linked glycosylation which is fundamental for proper protein folding and stability.
Biological function summary

GPSN2 is involved in the biosynthetic machinery as part of the oligosaccharyltransferase complex. This complex is responsible for the transfer of a glycan group to nascent polypeptides in the endoplasmic reticulum. GPSN2 contributes to efficient protein maturation ensuring that proteins acquire their correct conformations and functions. Its presence in this complex indicates important cell functions especially those involving protein processing and trafficking.

Pathways

GPSN2 takes part in the protein glycosylation pathway. This pathway is fundamental for various aspects of cell physiology including cell-cell communication and immune response. GPSN2 interacts with other proteins like STT3A and STT3B which are also components of the oligosaccharyltransferase complex. These interactions highlight its role in the glycosylation pathway influencing cellular homeostasis and signaling.

GPSN2 is associated with congenital disorders of glycosylation (CDG) and liver fibrosis. Structural abnormalities in GPSN2 can lead to CDG characterized by a wide range of developmental and metabolic issues. Additionally GPSN2's involvement in liver fibrosis links it to aberrant protein glycosylation which can exacerbate the progression of this liver disease. In these disorders GPSN2 often interacts with other glycosylation-related proteins such as the aforementioned STT3A further impacting disease development and progression.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com