AB257736

Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell lysate

Name: Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell lysate (ab257736) | Abcam
Brand: Abcam Limited
SKU: AB257736

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TFAP2A KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2.

View Alternative Names

AP-2, AP-2 transcription factor, AP2-alpha, AP2A_HUMAN, AP2TF, Activating enhancer-binding protein 2-alpha, Activator protein 2, BOFS, FLJ51761, TFAP 2, TFAP 2A, Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha), Transcription factor AP-2-alpha

3 Images

Lab

Western blot - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell lysate (AB257736)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : TFAP2A knockout HeLa cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab108311 observed at 48 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab108311 Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] was shown to specifically react with Transcription factor AP-2-alpha in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265122 (knockout cell lysate ab257736) was used. Wild-type and Transcription factor AP-2-alpha knockout samples were subjected to SDS-PAGE. ab108311 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (<a href='/en-us/products/primary-antibodies/transcription-factor-ap-2-alpha-antibody-epr26882-ab108311'>ab108311</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

TFAP2A knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-tfap2a-transcription-factor-ap-2-alpha-knockout-hela-cell-line-ab265122'>ab265122</a>)

Predicted band size: 122 kDa,125 kDa,32 kDa,39 kDa,40 kDa,41 kDa,42 kDa,45 kDa,48 kDa,74 kDa,77 kDa,83 kDa,97 kDa

Observed band size: 100 kDa,120 kDa,125 kDa,150 kDa,31 kDa,40 kDa,41 kDa,42 kDa,45 kDa,48 kDa,50 kDa,70 kDa,74 kDa,75 kDa,77 kDa,95 kDa

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Sanger Sequencing - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell lysate (AB257736)

Sanger seq

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Sanger Sequencing - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell lysate (AB257736)

Allele-1 : 1 bp insertion in exon 2

Sanger seq

Lab

Sanger Sequencing - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell lysate (AB257736)

Allele-2 : Insertion of the selection cassette in exon 2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2.

Disease

Adenocarcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name

TFAP2A

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing, Western blot

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Transcription factor AP-2-alpha also known as TFAP2A or AP-2 is a protein acting as an important regulator in cellular processes. It weighs approximately 52 kDa. TFAP2A is known for its role in binding specific DNA sequences to regulate target gene transcription. It expresses highly in neural crest-derived tissues as well as in the placenta breast and skin. This transcription factor plays an essential role in embryonic development cell proliferation and differentiation.

Biological function summary

TFAP2A contributes significantly to the regulation of gene expression involved in essential developmental and cellular processes. It acts as part of a complex with other transcription factors allowing it to coordinate the expression of a variety of genes linked to cell growth and apoptosis. AP-2 also regulates genes involved in neural development and skin morphogenesis. Its interaction with other factors such as AP-2γ and AP-2β enhances its functionality indicating a complex mechanism of action.

Pathways

TFAP2A engages in critical signalling circuits such as the MAPK and Wnt pathways which are essential for cell cycle regulation and embryonic development. Within these pathways related proteins like AP-2γ and AP-594 interact with TFAP2A further illustrating its role in signal transduction. These relationships highlight the importance of TFAP2A in orchestrating cellular responses to growth signals and environmental cues.

TFAP2A has links to a range of pathological conditions including cancer and neural crest-related disorders. In melanoma for example TFAP2A's regulatory role affects genes associated with tumor growth and metastasis. The protein's interaction with other transcription factors like AP-647 may influence cancer progression and therapy response. Additionally the dysregulation of TFAP2A affects neural crest development potentially leading to congenital disorders. Understanding these interactions assists in unraveling the molecular basis of such diseases and informs therapeutic strategies.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell lysate

Western blot - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell lysate (AB257736)

Sanger Sequencing - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell lysate (AB257736)

Sanger Sequencing - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell lysate (AB257736)

Key facts

Cell type

Species or organism

Tissue

Knockout validation

Mutation description

Disease

Reactivity data

Product details

What's included?

Properties and storage information

Gene name

Gene editing type

Gene editing method

Knockout validation

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Quality control

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

Product protocols

Complementary Products

Primary Antibodies

Proteins & Peptides

Proteins & Peptides

Primary Antibodies

Product promise