TFAP2A KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2.
Alternative names
AP-2, AP-2 transcription factor, AP2-alpha, AP2A_HUMAN, AP2TF, Activating enhancer-binding protein 2-alpha, Activator protein 2, BOFS, FLJ51761, TFAP 2, TFAP 2A, Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha), Transcription factor AP-2-alpha
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TFAP2A KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- TFAP2A
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Transcription factor AP-2-alpha also known as TFAP2A or AP-2 is a protein acting as an important regulator in cellular processes. It weighs approximately 52 kDa. TFAP2A is known for its role in binding specific DNA sequences to regulate target gene transcription. It expresses highly in neural crest-derived tissues as well as in the placenta breast and skin. This transcription factor plays an essential role in embryonic development cell proliferation and differentiation.
- Biological function summary
TFAP2A contributes significantly to the regulation of gene expression involved in essential developmental and cellular processes. It acts as part of a complex with other transcription factors allowing it to coordinate the expression of a variety of genes linked to cell growth and apoptosis. AP-2 also regulates genes involved in neural development and skin morphogenesis. Its interaction with other factors such as AP-2γ and AP-2β enhances its functionality indicating a complex mechanism of action.
- Pathways
TFAP2A engages in critical signalling circuits such as the MAPK and Wnt pathways which are essential for cell cycle regulation and embryonic development. Within these pathways related proteins like AP-2γ and AP-594 interact with TFAP2A further illustrating its role in signal transduction. These relationships highlight the importance of TFAP2A in orchestrating cellular responses to growth signals and environmental cues.
- Associated diseases and disorders
TFAP2A has links to a range of pathological conditions including cancer and neural crest-related disorders. In melanoma for example TFAP2A's regulatory role affects genes associated with tumor growth and metastasis. The protein's interaction with other transcription factors like AP-647 may influence cancer progression and therapy response. Additionally the dysregulation of TFAP2A affects neural crest development potentially leading to congenital disorders. Understanding these interactions assists in unraveling the molecular basis of such diseases and informs therapeutic strategies.
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3 product images
Western blot - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell lysate (ab257736)
Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: TFAP2A knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] ab108311 observed at 48 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] ab108311 Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] was shown to specifically react with Transcription factor AP-2-alpha in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line ab265122 (knockout cell lysate ab257736) was used. Wild-type and Transcription factor AP-2-alpha knockout samples were subjected to SDS-PAGE. Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] ab108311 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] ab108311) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: TFAP2A knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line (Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line ab265122)
Performed under reducing conditions.
Predicted band size: 122 kDa, 125 kDa, 32 kDa, 39 kDa, 40 kDa, 41 kDa, 42 kDa, 45 kDa, 48 kDa, 74 kDa, 77 kDa, 83 kDa, 97 kDa
Observed band size: 100 kDa, 120 kDa, 125 kDa, 150 kDa, 31 kDa, 40 kDa, 41 kDa, 42 kDa, 45 kDa, 48 kDa, 50 kDa, 70 kDa, 74 kDa, 75 kDa, 77 kDa, 95 kDa
Sanger Sequencing - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell lysate (ab257736)
Allele-1: 1 bp insertion in exon 2
Sanger Sequencing - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell lysate (ab257736)
Allele-2: Insertion of the selection cassette in exon 2
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