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AB269671

Human TGFB1 (TGF beta 1) knockout A549 cell lysate

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TGFB1 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 99.45%.
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Western blot - Human TGFB1 (TGF beta 1) knockout A549 cell lysate (AB269671)
  • WB

Lab

Western blot - Human TGFB1 (TGF beta 1) knockout A549 cell lysate (AB269671)

Lane 1 : Wild-type A549 cell lysate 20 μg
Lane 2 : TGFB1 knockout A549 cell lysate 20 μg
Lane 3 : K562 cell lysate 20 μg
Lane 4 : SH-SY5Y cell lysate 20 μg

Lanes 1 - 4 : Merged signal (red and green). Green - ab215715 observed at 48 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab215715 was shown to react with TGF beta 1 in wild-type A549 cells in Western blot with loss of signal observed in TGFB1 knockout cell line ab269509 (TGFB1 knockout cell lysate ab269671). Wild-type A549 and TGFB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab215715 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-TGF beta 1 antibody [EPR21143] (<a href='/en-us/products/primary-antibodies/tgf-beta-1-antibody-epr21143-ab215715'>ab215715</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

TGFB1 knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human TGFB1 (TGF beta 1) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-tgfb1-tgf-beta-1-knockout-a549-cell-line-ab269509'>ab269509</a>)

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 44 kDa

Observed band size: 48 kDa

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Western blot - Human TGFB1 (TGF beta 1) knockout A549 cell lysate (AB269671)
  • WB

Lab

Western blot - Human TGFB1 (TGF beta 1) knockout A549 cell lysate (AB269671)

Lane 1 : Wild-type A549 cell lysate 20 μg
Lane 2 : TGFB1 knockout A549 cell lysate 20 μg
Lane 3 : K562 cell lysate 20 μg
Lane 4 : SH-SY5Y cell lysate 20 μg

Lanes 1 - 4 : Merged signal (red and green). Green - ab179695 observed at 48 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab179695 was shown to react with TGF beta in wild-type A549 cells in Western blot with loss of signal observed in TGFB1 knockout cell line ab269509 (TGFB1 knockout cell lysate ab269671). Wild-type A549 and TGFB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab179695 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-TGF beta 1 antibody [EPR18163] (<a href='/en-us/products/primary-antibodies/tgf-beta-1-antibody-epr18163-ab179695'>ab179695</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Western blot - Human TGFB1 (TGF beta 1) knockout A549 cell lysate (ab269671) at 20 µg

Lane 2:

TGFB1 knockout A549 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 44 kDa

Observed band size: 48 kDa

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Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 99.45%

Disease

Carcinoma

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Complementary Products

Primary Antibodies

AB215715

Anti-TGF beta 1 antibody [EPR21143]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-TGF beta 1 antibody [EPR21143] (AB215715)

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  • 6
Proteins & Peptides

AB50036

Recombinant human TGF beta 1 protein (Active)

Bioactive

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Assay Kits

AB100647

Human TGF beta 1 ELISA Kit

Sandwich ELISA - Human TGF beta 1 ELISA Kit (AB100647)

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  • 2
Proteins & Peptides

AB90928

Native human Plasmin protein (Active)

SDS-PAGE - Native human Plasmin protein (Active) (AB90928)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
TGFB1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com