JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB263799

Human TGFB1 (TGF beta 1) knockout HeLa cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

TGFB1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

View Alternative Names

CED, Cartilage-inducing factor, DPD1, Differentiation inhibiting factor, LAP, Latency-associated peptide, Prepro transforming growth factor beta 1, TGF beta, TGF-beta 1 protein, TGF-beta-1, TGF-beta-5, TGFB, TGFB1_HUMAN, Transforming Growth Factor b1, Transforming Growth Factor beta-1, Transforming Growth Factor-闁1, Transforming growth factor beta 1a

2 Images
Western blot - Human TGFB1 (TGF beta 1) knockout HeLa cell lysate (AB263799)
  • WB

Lab

Western blot - Human TGFB1 (TGF beta 1) knockout HeLa cell lysate (AB263799)

Lane 1 : A549 cell lysate (20 μg)

Lane 2 : K562 cell lysate (20 μg)

Lane 3 : Wild-type HeLa cell lysate (20 μg)

Lane 4 : TGF beta 1 knockout HeLa cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab179695 observed at 50 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab179695 was shown to react with TGF beta 1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255439 (knockout cell lysate ab263799) was used. Wild-type and TGF beta 1 knockout samples were subjected to SDS-PAGE. ab179695 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TGF beta 1 antibody [EPR18163] (<a href='/en-us/products/primary-antibodies/tgf-beta-1-antibody-epr18163-ab179695'>ab179695</a>) at 1/1000 dilution

Lane 1:

A549 cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 2:

Western blot - Human TGFB1 (TGF beta 1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-tgfb1-tgf-beta-1-knockout-hela-cell-line-ab255439'>ab255439</a>)

Lane 3:

Wild-type HeLa cell lysate at 20 µg

Lane 4:

TGF beta 1 knockout HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 37 kDa,50 kDa

false

Sanger Sequencing - Human TGFB1 (TGF beta 1) knockout HeLa cell lysate (AB263799)
  • Sanger seq

Unknown

Sanger Sequencing - Human TGFB1 (TGF beta 1) knockout HeLa cell lysate (AB263799)

Homozygous : 1 bp deletion in exon 1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

Disease

Adenocarcinoma

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab263799-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human TGFB1 knockout HeLa cell lysate", "number":"AB263799-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB263799-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
TGFB1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB215715

Anti-TGF beta 1 antibody [EPR21143]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-TGF beta 1 antibody [EPR21143] (AB215715)

  • 5
  • 6
Proteins & Peptides

AB50036

Recombinant human TGF beta 1 protein (Active)

Bioactive

  • 0
  • 0
Assay Kits

AB100647

Human TGF beta 1 ELISA Kit

Sandwich ELISA - Human TGF beta 1 ELISA Kit (AB100647)

  • 5
  • 2
Proteins & Peptides

AB90928

Native human Plasmin protein (Active)

SDS-PAGE - Native human Plasmin protein (Active) (AB90928)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com