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AB261672

Human TGFBR2 (TGF beta Receptor II) knockout A549 cell lysate

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TGFBR2 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 97% knockout.

View Alternative Names

AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TGF beta receptor type IIB, TGF-beta receptor type II, TGF-beta receptor type-2, TGF-beta type II receptor, TGF-beta-R2, TGFB R2, TGFR2_HUMAN, TGFbeta - RII, TbetaR-II, Transforming growth factor beta receptor II, Transforming growth factor beta receptor type IIC, Transforming growth factor, beta receptor II (70/80kDa), Transforming growth factor-beta receptor type II, transforming growth factor, beta receptor II alpha, transforming growth factor, beta receptor II beta, transforming growth factor, beta receptor II delta, transforming growth factor, beta receptor II epsilon, transforming growth factor, beta receptor II gamma

2 Images
Western blot - Human TGFBR2 (TGF beta Receptor II) knockout A549 cell lysate (AB261672)
  • WB

Lab

Western blot - Human TGFBR2 (TGF beta Receptor II) knockout A549 cell lysate (AB261672)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 2 : TGFBR2 knockout A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate 20 ug

Lane 4 : HT-1080 whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab184948 observed at 80 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab184948 was shown to specifically react with in wild-type A549 cells as signal was lost in TGFBR2 knockout cell line ab261863 (knockout cell lysate ab261672). Wild-type and TGFBR2 knockout samples were subjected to SDS-PAGE. ab184948 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TGF beta Receptor II antibody [EPR14673] (<a href='/en-us/products/primary-antibodies/tgf-beta-receptor-ii-antibody-epr14673-ab184948'>ab184948</a>) at 1/2000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

TGFBR2 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human TGFBR2 (TGF beta Receptor II) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-tgfbr2-tgf-beta-receptor-ii-knockout-a549-cell-line-ab261863'>ab261863</a>)

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

HT-1080 whole cell lysate at 20 µg

Predicted band size: 65 kDa

Observed band size: 80 kDa

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Next Generation Sequencing - Human TGFBR2 (TGF beta Receptor II) knockout A549 cell lysate (AB261672)
  • NGS

Lab

Next Generation Sequencing - Human TGFBR2 (TGF beta Receptor II) knockout A549 cell lysate (AB261672)

X = 1 bp insertion

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 97% knockout

Disease

Carcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
TGFBR2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Complementary Products

Cell Lines & Lysates

AB261863

Human TGFBR2 (TGF beta Receptor II) knockout A549 cell line

Advanced Validation

Western blot - Human TGFBR2 (TGF beta Receptor II) knockout A549 cell line (AB261863)

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Proteins & Peptides

AB316468

Recombinant Mouse TGF beta Receptor II Protein Standard (His tag)

Sandwich ELISA - Recombinant Mouse TGF beta Receptor II Protein Standard (His tag) (AB316468)

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Primary Antibodies

AB184948

Anti-TGF beta Receptor II antibody [EPR14673]

RabMAb|Recombinant|KO Validated

Western blot - Anti-TGF beta Receptor II antibody [EPR14673] (AB184948)

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Assay Kits

AB193715

Human TGF beta R2 ELISA Kit

Sandwich ELISA - Human TGF beta R2 ELISA Kit (AB193715)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com