Human THAP7 knockout HeLa cell lysate
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THAP7 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.
View Alternative Names
1810004B07Rik, THAP domain containing 7, THAP domain-containing protein 7, THAP7_HUMAN
- Sanger seq
Unknown
Sanger Sequencing - Human THAP7 knockout HeLa cell lysate (AB263384)
Allele-1 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human THAP7 knockout HeLa cell lysate (AB263384)
Allele-2 : Insertion of the selection cassette in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The actions of THAP7 involve regulating gene expression and cell cycle control. It interacts with other proteins to form transcriptional repressor complexes impacting gene silencing. These activities play roles in cell proliferation and differentiation. THAP7's involvement in these fundamental processes indicates its importance in maintaining cellular homeostasis.
Pathways
THAP7 participates in cell cycle regulation and transcription pathways. It has associations with the retinoblastoma pathway collaborating with proteins like E2F transcription factors to influence cell cycle progression. These interactions show that THAP7 contributes to the control of cellular division and proliferation impacting the transition through cell cycle phases.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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