Human THOC3 knockout HeLa cell lysate
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THOC3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.
View Alternative Names
TEX1 homolog, THO complex subunit 3, THOC3_HUMAN, Tho3, hTREX45
- Sanger seq
Unknown
Sanger Sequencing - Human THOC3 knockout HeLa cell lysate (AB263385)
Allele-1 : 2 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human THOC3 knockout HeLa cell lysate (AB263385)
Allele-2 : 1 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
THOC3 functions as part of the TREX complex which couples transcription with mRNA processing and export. The TREX complex is responsible for enhancing the efficiency of transcription elongation and mRNA release enabling the proper export of mRNA to the cytoplasm. THOC3 along with other core components of the complex maintains the integrity of mRNA ensuring only correctly processed mRNA exits the nucleus for translation.
Pathways
THOC3 is a critical participant in the mRNA export pathway an important aspect of gene expression regulation. This protein interacts with other members of the TREX complex such as THOC1 and THOC2 facilitating mRNA export and ensuring seamless communication between transcription and translation processes. Besides THOC3 ties into the larger gene expression and regulation network supporting cellular growth and function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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